We narrowed to 1,876 results for: IRES-GFP

1. pX458-Ef1a-Cas9-H2B-GFP

   Plasmid

   #159654

   Purpose

   Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

   Plasmid

   #190153

   Purpose

   Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp2 (Vamp2 Mouse)
   

   Use

   AAV

   Tags

   P2AT2A-EGFP-caax

   Expression

   Mammalian

   Mutation

   Truncation that includes only AA #1-94

   Promoter

   MBP

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

   Plasmid

   #174895

   Purpose

   amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   102TAG 150TAA in sfGFP

   Promoter

   EF1

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

   Plasmid

   #190154

   Purpose

   Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp3 (Vamp2 Rat)
   

   Use

   AAV

   Tags

   P2AT2A-EGFP-caax

   Expression

   Mammalian

   Mutation

   Truncation that includes only rat Vamp3 AA #1-81

   Promoter

   MBP

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-Neo-GFP/Flag-Gata5

   Plasmid

   #105508

   Purpose

   MSCV-driven retroviral Gata5 expression (Flag-tagged)

   Depositor

   Christopher Vakoc

   Article

   Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

   Insert

   Gata5 (Gata5 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMXs-puro GFP-p62 K7A, D69A

   Plasmid

   #38281

   Depositor

   Noboru Mizushima

   Article

   Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

   Insert

   Sequestosome-1 (Sqstm1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed K7A, D69A

   Promoter

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pCS2 stabilized mutant Beta Catenin-GFP

   Plasmid

   #29684

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   beta catenin (ctnnb1.L Frog)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S33A, S37A, T41A

   Promoter

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

   Plasmid

   #159658

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

   Plasmid

   #159657

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

    Plasmid

    #159664

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

    Plasmid

    #159663

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

    Plasmid

    #159662

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

    Plasmid

    #159656

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBabe-GFP-IKBalpha-mut (super repressor)

    Plasmid

    #15264

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKB alpha (NFKBIA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Serine 32 to Alanine, Serine 36 to Alanine

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCT-CMV-copGFP-Polß(L301R/V303R/V306R)-puro

    Plasmid

    #128666

    Purpose

    mammalian expression of copGFP-Polß(L301R/V303R/V306R: TM) protein with puromcyin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    copGFP

    Expression

    Mammalian

    Mutation

    L301R/V303R/V306R

    Promoter

    CMV

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXs-puro GFP-p62 D337, 338, 339A

    Plasmid

    #38280

    Depositor

    Noboru Mizushima

    Article

    Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

    Insert

    Sequestosome-1 (Sqstm1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed D337A, D338A, D339A

    Promoter

    Available since

    Sept. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pLenti-Zyxin-Full Length (aa1-564)-EGFP

    Plasmid

    #187692

    Purpose

    Visualization of Zyxin

    Depositor

    Mary Beckerle

    Article

    Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

    Insert

    Zyxin (Zyx Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Flag-HA-GFP

    Plasmid

    #22612

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-GATA3gfp

    Plasmid

    #83819

    Purpose

    Mammalian expression of GATA3 fused to GFP

    Depositor

    Douglas Engel

    Insert

    GATA3 (Gata3 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ii-Str_ssSBP-EGFP

    Plasmid

    #65277

    Purpose

    to synchronize transport of secretory soluble GFP from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    EGFP
    

    Use

    Tags

    IL-2 signal sequence and Streptavidin Binding Pr…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ST-SBP-EGFP_puromycin

    Plasmid

    #65270

    Purpose

    ST reporter only ( no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA_Lifeact-GFP_NLS-mCherry

    Plasmid

    #69058

    Purpose

    Dual Fluorescent Reporter for F-actin and Nuclei

    Depositor

    Olivier Pertz

    Insert

    LifeAct-GFP_IRES_NLS-mCherry
    

    Use

    Tags

    Lifeact-GFP, NLS-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EGFP-p300

    Plasmid

    #191764

    Purpose

    Expression of EGFP fused to core p300 histone acetyltransferase

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    FRB-EGFP-p300 core (EP300 Human)
    

    Use

    Tags

    EGFP (N-terminal of p300 core) and FRB (N-termina…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAG1344 EGFP-FIREtag

    Plasmid

    #205165

    Purpose

    Expresses EGFP-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    EGFP-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Ii-Str_SBP-EGFP-Ecadherin

    Plasmid

    #65288

    Purpose

    synchronize trafficking of Ecadherin from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Ii-Str_SBP-EGFP-GPI

    Plasmid

    #65296

    Purpose

    synchronize trafficking of EGFP-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Use

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. VSVGwt-SBP-EGFP_puromycin

    Plasmid

    #65302

    Purpose

    VSVG reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. flGalT-SBP-EGFP_puromycin

    Plasmid

    #65275

    Purpose

    GalT reporter only ( no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    galactosidase
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 8024 GFP_Kras_G12C_2A_puro

    Plasmid

    #64373

    Purpose

    This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2A oncogene along with puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    mouse Kras2A (Kras Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    changed glycine 12 to cysteine

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 8027_GFP-KrasG12C_2B_puro

    Plasmid

    #64372

    Purpose

    This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    mouse Kras2B (Kras Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    changed glycine 12 to cysteine

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge