We narrowed to 155,989 results for: ins
-
Plasmid#197085PurposeThis plasmid contains the coding sequence for signaling peptide M5, which contains the YESP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M5, which contains the YESP signaling motif.
ExpressionMammalianAvailable SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M6_ITYAAV
Plasmid#197086PurposeThis plasmid contains the coding sequence for signaling peptide M6, which contains the ITYAAV signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M6, which contains the ITYAAV signaling motif.
ExpressionMammalianAvailable SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M7_PVQP
Plasmid#197087PurposeThis plasmid contains the coding sequence for signaling peptide M7, which contains the PVQP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M7, which contains the PVQP signaling motif.
ExpressionMammalianAvailable SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M8_RSTKP
Plasmid#197088PurposeThis plasmid contains the coding sequence for signaling peptide M8, which contains the RSTKP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M8, which contains the RSTKP signaling motif.
ExpressionMammalianAvailable SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M9_PVQE
Plasmid#197091PurposeThis plasmid contains the coding sequence for signaling peptide M9, which contains the PVQE signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M9, which contains the PVQE signaling motif.
ExpressionMammalianAvailable SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS110
Plasmid#215674PurposeSplit hygromycinR landing pad insertion plasmid for ChrIIIDepositorInsert5'HA + synthetic guide site3 + 3'ΔHygR:unc-54 3' UTR + lox2272 + SEC + lox2272 + 3'HA
UseCRISPR and Cre/LoxExpressionWormMutation3' ∆HYGR is promoterless and encodes aa60-341Available SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUiLMO2W
Plasmid#129480Purpose3rd-generation lentiviral vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemicalDepositorInsertiLMO2
UseLentiviralExpressionMammalianPromoterhuman ubiquitin CAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-iLMO2
Plasmid#129474PurposeMammalian expression vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemicalDepositorInsertiLMO2
ExpressionMammalianPromoterCMVAvailable SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-null (BPK1077)
Plasmid#171128PurposePlasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.DepositorInsertpCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
UseCRISPRExpressionBacterial and MammalianPromoterpCMV and T7Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc180
Plasmid#161942PurposeCarries Golden Gate substitution insert for acVHH-assisted bisection mappingDepositorInsertpSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
ExpressionBacterialPromoterPBADAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc226
Plasmid#161944PurposeContains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmidDepositorInsertpSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
ExpressionBacterialPromoterPBADAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
mPLD2-PX (62-192)
Plasmid#119128PurposeBacterial expression of human phox homology (PX) domain, mPLD2-PX (62-192)DepositorInsertmPLD2-PX (62-192)
TagsGSTExpressionBacterialPromotertacAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab8 DN
Plasmid#58451Purposeexpression of drosophila YFP Rab8 dominant negative mutantDepositorAvailable SinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab2 DN
Plasmid#58445Purposeexpression of drosophila YFP-Rab2 dominant negative mutantDepositorAvailable SinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab2 CA
Plasmid#58443Purposeexpression of drosophila YFP-Rab2 constitutively active mutantDepositorAvailable SinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab14 DN
Plasmid#37715DepositorInsertRab14 (Rab14 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS49N (dominant negative)PromoterGAL4Available SinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
SNX26-PX (56-172)
Plasmid#119105PurposeBacterial expression of human phox homology (PX) domain, SNX26-PX (56-172)DepositorInsertSNX26-PX (56-172)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
SNX28-PX (1-124)
Plasmid#119107PurposeBacterial expression of human phox homology (PX) domain, SNX28-PX (1-124)DepositorInsertSNX28-PX (1-124)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
SNX34-PX (1-130)
Plasmid#119113PurposeBacterial expression of human phox homology (PX) domain, SNX34-PX (1-130)DepositorInsertSNX34-PX (1-130)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
IRAS-PX (13-124)
Plasmid#119118PurposeBacterial expression of human phox homology (PX) domain, IRAS-PX (13-124)DepositorInsertIRAS-PX (13-124)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pSFFV-SpyTag-sfCherry2(11)-TagBFP
Plasmid#117485PurposeExpresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cellsDepositorInsertSpyTag002-GG-sfCherry2(11)
UseLentiviralTagsTagBFPExpressionMammalianPromoterSFFVAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)
Plasmid#181792PurposeExpresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher
Plasmid#117656PurposeBacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.DepositorInsertssfCherry2(1-10)
sfCherry2(11)-SpyCatcher002
ExpressionBacterialPromoterT7Available SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-Arl13b12
Plasmid#40872DepositorAvailable SinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
DJ-1 TEV site pET15b
Plasmid#60687PurposeRecombinant protein expression of Park7DepositorInsertDJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationTEV site was inserted (see comments below).PromoterT7Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-10H-SIII
Plasmid#175444PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneTags3C-10His-TwinStrep and BiP Secretion PeptideExpressionInsectPromoterFused Actin-HSP70Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-Arl13b11
Plasmid#40873DepositorInsertArl13b (Arl13b Mouse)
TagsHis and V5ExpressionMammalianMutationno stop codonPromoterCMVAvailable SinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
SNX10-PX (1-201)
Plasmid#119091PurposeBacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)DepositorAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)
Plasmid#134341PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIC3
TagsNLS(SV40)ExpressionMammalianPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)
Plasmid#134337PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA16_Efa
TagsNLS(SV40)ExpressionMammalianPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only