We narrowed to 7,219 results for: ALP

1. NES-mCherry-PH-BTKx1

   Plasmid

   #183653

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Frog, Human)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-mCherry

   Expression

   Mammalian

   Mutation

   Amino acids 2-170

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA-HIF2α P405A/N847A Δ450-572-pBabe-Puro

   Plasmid

   #25936

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 405 to Alanin…

   Promoter

   Available since

   Sept. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TRUPATH Triple Gai1

   Plasmid

   #196048

   Purpose

   Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai1-RLuc8 (GNAI1 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TRUPATH Triple (bidirectional) GasS

   Plasmid

   #196055

   Purpose

   Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphasS-RLuc8
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR223-CAMK2A

   Plasmid

   #23408

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   CAMK2A (CAMK2A Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Capping Protein

   Plasmid

   #89950

   Purpose

   F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.

   Depositor

   Guillaume Romet-Lemonne, Antoine Jégou

   Article

   Shekhar et al Nat Commun. 2015 Nov 13;6:8730. doi: 10.

   Inserts

   F-actin-capping protein subunit alpha-1 (Capza1 Mouse)
   F-actin-capping protein subunit beta (Capzb Mouse)
   

   Use

   Tags

   6*His

   Expression

   Bacterial

   Mutation

   V222I

   Promoter

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223_HIF1A_WT

   Plasmid

   #82129

   Purpose

   Gateway Donor vector containing HIF1A , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   HIF1A (HIF1A Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223-PIK3CA

   Plasmid

   #23466

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   A1035V

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pDONR223_ERO1L_WT

   Plasmid

   #81797

   Purpose

   Gateway Donor vector containing ERO1L , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   ERO1L (ERO1A Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KRD9_HBA1(HBA1reg-HBB)

    Plasmid

    #232402

    Purpose

    AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank HBA1 cassette. HAs are ~400bp each.

    Depositor

    Kyle Cromer, Matthew Porteus

    Article

    Cromer et al Nat Med. 2021 Apr;27(4):677-687. doi: 10

    Insert

    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. KRD2_HBA1(HBA1reg-UbC-GFP)

    Plasmid

    #232401

    Purpose

    AAV production plasmid for HBA1 WGR vector from Fig. 1 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBA1 UTRs flank UbC-GFP cassette; GFP is followed by BGH polyA. HAs are ~400bp each.

    Depositor

    Kyle Cromer, Matthew Porteus

    Article

    Cromer et al Nat Med. 2021 Apr;27(4):677-687. doi: 10

    Insert

    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLViN-iRFP670-α-cateninA+ΔβH

    Plasmid

    #229707

    Purpose

    Lentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    amino acids 670-673, RAIM--> GSGS

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

    Plasmid

    #209199

    Purpose

    Mutagenesis of Kcnma1

    Depositor

    Larry Zweifel

    Article

    Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

    Insert

    Kcnma1 (Kcnma1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Anti-Slo1 K+ channel [L6/60R-1]

    Plasmid

    #206694

    Purpose

    Mammalian Expression Plasmid of anti-Slo1 K+ channel (Mouse). Derived from hybridoma L6/60-1.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Slo1 K+ channel (Mus musculus) recombinant Mouse monoclonal antibody (Kcnma1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TRUPATH Triple Gai3

    Plasmid

    #196050

    Purpose

    Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai3-RLuc8 (GNAI3 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TRUPATH Triple Ga12

    Plasmid

    #196061

    Purpose

    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRUPATH Triple Ga13

    Plasmid

    #196062

    Purpose

    Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha13-RLuc8 (GNA13 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KRD22_HBA1(HBA1reg-HBB-longHAs)

    Plasmid

    #232403

    Purpose

    AAV production plasmid for HBA1 long HAs vector from Figs. 3-6 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank full HBA1-2A-YFP cassette. HAs are ~900bp

    Depositor

    Kyle Cromer, Matthew Porteus

    Article

    Cromer et al Nat Med. 2021 Apr;27(4):677-687. doi: 10

    Inserts

    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Human Hemoglobin Subunit Beta (HBB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HisGB1-Fusion + CBP TAZ1(340-439)

    Plasmid

    #173762

    Purpose

    Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HisGB1-FusionL21A + CBP TAZ1(340-439)

    Plasmid

    #173763

    Purpose

    Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HIsGB1-FusionL63A + CBP TAZ1(340-439)

    Plasmid

    #173764

    Purpose

    Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TRUPATH Triple Gai2

    Plasmid

    #196049

    Purpose

    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TRUPATH Triple Ga11

    Plasmid

    #196059

    Purpose

    Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha11-RLuc8 (GNA11 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TRUPATH Triple Gagust

    Plasmid

    #196054

    Purpose

    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TRUPATH Triple Ga15

    Plasmid

    #196060

    Purpose

    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PB513B-1/TRE-hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ-EF1α-Puro

    Plasmid

    #199550

    Purpose

    PiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBP-γ) under control of the TRE/PCMVmin promoter

    Depositor

    Masamichi Kamihira

    Article

    Kitano et al Cells. 2022 Apr 1;11(7):1194. doi: 10.33

    Insert

    hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ (HNF1A, FOXA2, CEBPB, CEBPA, CEBPG Human)
    

    Use

    Piggybac transposon vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE+CMVmin promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TFORF2975

    Plasmid

    #144451

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NAT10 (NAT10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TFORF2510

    Plasmid

    #144032

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NAT10 (NAT10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEMS1499

    Plasmid

    #29247

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple28 (CCL27 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pEMS1498

    Plasmid

    #29246

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple27 (CCL27 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pEMS1500

    Plasmid

    #29248

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple29 (CCL27 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pHIV-Luc-ZsGreen

    Plasmid

    #39196

    Depositor

    Bryan Welm

    Inserts

    Firefly Luciferase Luc2P
    ZsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha and EF1-alpha

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHIV-Luciferase

    Plasmid

    #21375

    Purpose

    self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

    Depositor

    Bryan Welm

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Gaq-LSS-SGFP2

    Plasmid

    #112951

    Purpose

    Galphaq tagged with LSS-SGFP2

    Depositor

    Dorus Gadella

    Insert

    Galphaq
    

    Use

    Tags

    LSS-SGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEYFP-C1-p85beta

    Plasmid

    #1408

    Depositor

    Lewis Cantley

    Insert

    PI3K regulatory subunit p85beta (Pik3r2 Mouse)
    

    Use

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

36. pRSV-PKI-v2

    Plasmid

    #45066

    Purpose

    Expression of PKI (PKA inhibitor)

    Depositor

    Richard Maurer

    Insert

    cAMP-dependent protein kinase inhibitor alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX-4T3-p85beta

    Plasmid

    #1406

    Depositor

    Lewis Cantley

    Insert

    PI3K regulatory subunit p85beta (Pik3r2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

38. pRSV-PKImut-v2

    Plasmid

    #45067

    Purpose

    Expression of intactive PKI (PKA inhibitor) mutant

    Depositor

    Richard Maurer

    Insert

    cAMP-dependent protein kinase inhibitor alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Arg 20 & 21 to Glycines

    Promoter

    RSV

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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39. pHCA/GAL4(848).ER

    Plasmid

    #108215

    Purpose

    Yeast expression vector for fusion of Gal4 AA 1-848 to hormone binding domain of estrogen receptor a

    Depositor

    Didier Picard

    Insert

    Gal4-ERalpha HBD
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERalpha HBD has G400V mutation

    Promoter

    ADH0

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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40. pGEXTK-Pak1 70-117

    Plasmid

    #12217

    Depositor

    Jonathan Chernoff

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    PBD (p21-binding domain), aa 70-117

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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41. pLKO-puro-hPPM1A

    Plasmid

    #185382

    Purpose

    For mammalian expression of shRNA: AGGGTAATGGGTTGCGATATG that targets human PPM1A

    Depositor

    Ayaz Najafov

    Insert

    PPM1A (PPM1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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42. pTUB:FLuc

    Plasmid

    #114578

    Purpose

    Constitutive expression of firefly luciferase in T. gondii using the alpha tubulin promoter; floxed pyrimethamine selectable marker; UPRT locus flanking sequence to direct integration

    Depositor

    David Sibley

    Insert

    Firefly Luciferase
    

    Use

    Cre/Lox and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    alpha tubulin T. gondii

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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43. pKPY115

    Plasmid

    #72669

    Purpose

    Encodes Thr251Gly-EcPheRS Under Arabinose-Inducible (PBAD) Control

    Depositor

    David Tirrell

    Insert

    Thr251Gly-EcPheRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Thr251Gly in Alpha Subunit

    Promoter

    PBAD

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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44. pLenti_PGK_Prkaa1

    Plasmid

    #204356

    Purpose

    Lentiviral plasmid, Expresses Prkaa1 under the control of the PGK promoter

    Depositor

    Günter Schneider

    Insert

    AMP-activated protein kinase alpha 1 (Prkaa1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. pT2/shαSMA/GFP4_Seq2/clone1

    Plasmid

    #201402

    Purpose

    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    alpha-SMA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. R777-E171 Hs.PIK3R1

    Plasmid

    #70455

    Purpose

    Gateway ORF clone of human PIK3R1 [NM_181523.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. R777-E172 Hs.PIK3R1-nostop

    Plasmid

    #70456

    Purpose

    Gateway ORF clone of human PIK3R1 [NM_181523.2] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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48. R777-E147 Hs.PAK1

    Plasmid

    #70431

    Purpose

    Gateway ORF clone of human PAK1 [NM_002576.4] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PAK1 (PAK1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid

    #217304

    Purpose

    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

    Depositor

    Luke Gilbert

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. R777-E148 Hs.PAK1-nostop

    Plasmid

    #70432

    Purpose

    Gateway ORF clone of human PAK1 [NM_002576.4] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PAK1 (PAK1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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