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Plasmid#73426PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.DepositorInsertPromoter 1E4 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP1E4 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-1B6-mCherry
Plasmid#73420PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.DepositorInsertPromoter 1B6 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP1B6 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-3H5-mCherry
Plasmid#73419PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.DepositorInsertPromoter 3H5 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP3H5 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-T7-Ntx-K49
Plasmid#58715PurposeExpresses in Escherichia coli T7-Notexin with the amino acid substitution D49KDepositorInsertNotexin
UseTagsT7 peptide tagExpressionBacterialMutationChanged Aspartic Acid 49 to LysinePromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK048
Plasmid#75230PurposeProduces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPro64 to Arg; Asp65 to His; Gln206 to Arg and cha…PromoterT7 and n/aAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pKSJ340
Plasmid#27125DepositorInsertColicin Ia + Ia immunity protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pMevT
Plasmid#17815DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-ack
Plasmid#62654PurposeArabinose inducible lambda red and anhydrotetracycline inducible sgRNA expressionDepositorInsertexo, beta, gam, sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZE27GFP
Plasmid#75452PurposePlasmid for constitutive expression of gfpDepositorInsertgfp
UseTagsExpressionBacterialMutationPromoterplacIq promoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
lacI-PT5-gusA-pBAV1k
Plasmid#30501DepositorInsertbeta-glucuronidase (gusA Escherichia coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-MBP-TEV-GG
Plasmid#215800PurposeCloning of target gene with N terminal His6 MBP tag and TEV CleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZE27MC3
Plasmid#75464PurposePlasmid for constitutive expression of mcherry-pdt3DepositorInsertmcherry-pdt#3
UseTagsExpressionBacterialMutationPromoterplacIq promoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
BioBrick pET-24a(+)
Plasmid#117802PurposeBioBrick pET vector for protein overexpression in E. coliDepositorTypeEmpty backboneUseEscherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB33eCPX
Plasmid#23336DepositorInsertEnhanced Circularly Permuted Outer Membrane Protein X
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-NUSA-TEV-GG
Plasmid#215798PurposeCloning of target gene with N terminal His6 NUSA tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAK461
Plasmid#140699PurposeNBαRb-IgG-FcDepositorInsertNBαRb-IgG-Fc
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
lacI-PT7-gusA-pBAV1k
Plasmid#30504DepositorInsertbeta-glucoronidase (gusA Escherichia coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDSG594
Plasmid#214221Purposecompetition strain 10 integrase plasmidDepositorInsertPhlF, A118
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CT-sfGFP-HN-GG
Plasmid#215803PurposeCloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pECJ3
Plasmid#75465PurposeToggle switch plasmid containing lacI-pdt3DepositorInsertlacI-pdt3
UseTagsExpressionBacterialMutationPromoterpLtetOAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNO41
Plasmid#101067PurposeBphP1-PpsR2 chromophore plasmid; ho1DepositorInsertho1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIT5_SL
Plasmid#173769PurposeBacterial genomic integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIT5_KT
Plasmid#173773PurposeBacterial genomic integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIT5_KH
Plasmid#173772PurposeBacterial genomic integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-THRX-TEV-GG
Plasmid#215796PurposeCloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFGL821-8
Plasmid#58224PurposeFor fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.DepositorInserthygromycin B phosphotransferase
UseTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSKDuet16
Plasmid#41684DepositorInsertNpuDnaE intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-GST-TEV-GG
Plasmid#215801PurposeCloning of target gene with N terminal His6 GST tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZE27GFP3
Plasmid#75456PurposePlasmid for constitutive expression of gfp-pdt3DepositorInsertgfp-pdt#3
UseTagsExpressionBacterialMutationPromoterplacIq promoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZE27GFP5
Plasmid#75458PurposePlasmid for constitutive expression of gfp-pdt5DepositorInsertgfp-pdt#5
UseTagsExpressionBacterialMutationPromoterplacIq promoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCYP29
Plasmid#212862PurposeExpresses the Streptomyces clavuligerus LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationPromoterPcymRCAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPro24(s)-gfp
Plasmid#17813DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationD234N compared to AF007834PromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUC-STS
Plasmid#35949DepositorInsertStilbene synthase
UseSynthetic BiologyTagsExpressionMutationPromoterLacPModAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
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FtsZ-Dendra2
Plasmid#92038PurposeExpress FtsZ-Dendra2DepositorInsertFtsZ
UseTagsDendra2ExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT122
Plasmid#31396DepositorInsertpJT122
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMAK462
Plasmid#140700PurposeNBαMs/Rt-IgG1-FcDepositorInsertNBαMs/Rt-IgG1-Fc
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMB413a
Plasmid#20058DepositorInsertSperm Whale Myoglobin
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
lacI-Ptac-gusA-pBAV1k
Plasmid#30503DepositorInsertbeta-glucuronidase (gusA Escherichia coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
araC-pBAD-gusA-pBAV1k
Plasmid#30502DepositorInsertbeta-glucuronidase (gusA Escherichia coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCYP01
Plasmid#212834PurposeExpresses the Rhizobium aethiopicum LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationPromoterPcymRCAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP15
Plasmid#212848PurposeExpresses the Micromonospora cremea LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationPromoterPcymRCAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLMB51
Plasmid#40083DepositorInsertsgusA
tauR
tau promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIT5_CL
Plasmid#173767PurposeBacterial genomic integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIT5_KH_VanR
Plasmid#173779PurposeBacterial genomic integration vector, VanR repressorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIT5_TL
Plasmid#173768PurposeBacterial genomic integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECJ3B
Plasmid#75466PurposeToggle switch plasmid containing lacI-pdt3BDepositorInsertlacI-pdt3B
UseTagsExpressionBacterialMutationPromoterpLtetOAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUB23-M-beta-gal
Plasmid#8816DepositorInsertbeta-gal
UseTagsUbiquitinExpressionYeastMutationM (methionine) at amino terminus of beta-galPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTGV-Express-CAT SMR5916
Plasmid#28039DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only