We narrowed to 1,197 results for: Aspa

1. pJK279

   Plasmid

   #71135

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes histidine-89 to aspartate; additional N-t…

   Promoter

   T7

   Available since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJK283

   Plasmid

   #71139

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H59N mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes histidine-59 to asparagine; additional N-…

   Promoter

   T7

   Available since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK282

   Plasmid

   #71138

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes histidine-59 to aspartate; additional N-t…

   Promoter

   T7

   Available since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAC-EsaR-D91G/V220A

   Plasmid

   #47648

   Purpose

   pAC-EsaR with a272g and c659t mutations in esaR

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

   Insert

   esaR-D91G/V220A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed Aspartic acid 91 to Glycine, and Valine 2…

   Promoter

   Plac

   Available since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

5. XE118 CAMKII-T286D-CS2+

   Plasmid

   #16736

   Depositor

   Randall Moon

   Article

   Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

   Insert

   CAMKII-T286D (Camk2a Rat)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   threonine 286 mutated to aspartate

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

6. mEmerald-CD81-10

   Plasmid

   #54031

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   He et al PLoS Pathog. 2013;9(10):e1003701. doi: 1

   Insert

   CD81 (CD81 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pRH2502

   Plasmid

   #84379

   Purpose

   Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

   Depositor

   Robert Husson

   Article

   Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

   Insert

   dcas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Aspartate at position 10 changed to Alanine, Hist…

   Promoter

   uv15tetO

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEYFP-NR1a

   Plasmid

   #17928

   Depositor

   Stefano Vicini

   Article

   Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

   Insert

   N-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRCT

   Plasmid

   #60621

   Purpose

   Plasmid encoding iCas9, tracrRNA and crRNAs

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti:FlipGFP-T2A-mCherry

    Plasmid

    #223198

    Purpose

    Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

    Depositor

    Yeh-Hsing Lao

    Article

    Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

    Insert

    FlipGFP-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMEH371 CD63 HA

    Plasmid

    #168220

    Purpose

    Mammalian expression of tetraspanin CD63 fused to a C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    CD63 (CD63 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PCS2-FlipGFP(Casp3 cleavage seq)

    Plasmid

    #124432

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq)
    

    Use

    Zebrafish (good expression)

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pARF1(T31N)-CFP

    Plasmid

    #11384

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 1 (ARF1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 31 to Asparagine.

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-NR1a

    Plasmid

    #17926

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (Grinl1a Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRRL MND WASp

    Plasmid

    #36248

    Depositor

    David Rawlings

    Article

    Astrakhan et al Blood. 2012 Mar 19.

    Insert

    WASp (WAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    MND

    Available since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2-mCherry-Rab11a (S25N)

    Plasmid

    #184036

    Purpose

    Expresses dominate negative mCherry Rab11a in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

    Insert

    Rab11
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Serine 25 changed to Asparagine

    Promoter

    CMV IE94

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pARF6(T27N)-CFP

    Plasmid

    #11386

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 6 (ARF6 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 27 to Asparagine.

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mTagBFP2-CD81-10

    Plasmid

    #55281

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    CD81 (CD81 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. GFP-rab9 DN

    Plasmid

    #12664

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB9 (Homo sapiens) (RAB9A Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    changed serine 21 to asparagine

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3/mArf1(T31N)-HA

    Plasmid

    #79411

    Purpose

    Expresses C-terminally HA-tagged Arf1(T31N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf1 (Arf1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 31 to Asparagine

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge