We narrowed to 12,386 results for: cel.2

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pBiFC-HA-hCaspase 2 FL (C303S)-VC155

Plasmid

#49257

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 (CASP2 Human)

Tags

HA and split Venus C

Expression

Mammalian

Mutation

C303S

Promoter

CMV

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
pLenti-TRS-Leader-SARS-CoV-2-EGFP

Plasmid

#171585

Purpose

Expression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporter

Depositor

Neville Sanjana

Article

Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

Insert

TRS-Leader-SARS-CoV-2-d2eGFP

Use

Lentiviral

Tags

2PEST AND NLS

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2 TetO(sh) mCh-Rs1-2

Plasmid

#24417

Purpose

Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Plasmid also known as pEntR3L2 TetO-mCh-Rs1.

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Rs1

Use

Entry vector

Tags

FLAG and mCherry-P2A

Mutation

D100A

Promoter

short TetO, miniCMV

Available Since

March 14, 2011

Availability

Academic Institutions and Nonprofits only

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pBiFC-flag-hCaspase 2 FL (C303S)-VN173

Plasmid

#49258

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 (CASP2 Human)

Tags

flag and split Venus N

Expression

Mammalian

Mutation

C303S

Promoter

CMV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pET28a 6xHis PreScission SARS-CoV-2 nsp13

Plasmid

#159390

Purpose

Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

Depositor

Tarun Kapoor

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression

Tags

His6 tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 BQ.1.1 Spike

Plasmid

#193710

Purpose

Expresses codon-optimized full length SARS-CoV-2 BQ.1.1 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BQ.1.1 Spike (S )

Expression

Mammalian

Mutation

Contains the following mutations: T19I, LPPA24S, …

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

Nov. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS SARS-CoV-2 BA.4.6 Spike

Plasmid

#190013

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.4.6 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BA.4.6 Spike (S )

Expression

Mammalian

Mutation

Contains the following mutations: T19I, LPPA24S, …

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

Sept. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS SARS-CoV-2 BA.2.75 Spike

Plasmid

#190012

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BA.2.75 Spike (S )

Expression

Mammalian

Mutation

Contains the BA.2 mutations (T19I, LPPA24S, G142D…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

Sept. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCellFree_G03 USF2

Plasmid

#67069

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

USF2 (USF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 SREBF2

Plasmid

#67129

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SREBF2 (SREBF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFDP2

Plasmid

#67088

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP2 (TFDP2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCAGGS SARS-CoV-2 BA.1 Spike

Plasmid

#185452

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.1 Spike

Depositor

Marceline Côté

Article

Chatterjee et al Cell Rep. 2022 Mar 1;38(9):110429. doi:

Insert

SARS-CoV-2 Spike BA.1 (S )

Expression

Mammalian

Mutation

Contains the following mutations: A67V, Δ69-70, T…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-GFP-CENPBpA-fixed-length-clone-2

Plasmid

#120180

Purpose

Randomly picked clone 2 of the pCMV-GFP-CENPBpA-fixed-length library

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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B52 (empty plasmid backbone to express 2 sgRNAs)

Plasmid

#100708

Purpose

Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

Plasmid

#128760

Purpose

Expresses PARN gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PARN sgRNA #2 (PARN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

Plasmid

#128757

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir193b-2

Plasmid

#46732

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir193b-2 (MIR193B Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir50-2

Plasmid

#46711

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir50-2

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir44-2

Plasmid

#46705

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir44-2

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir30-2

Plasmid

#46689

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir30-2 (MIR30A Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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