Addgene

We narrowed to 151,085 results for: des

Showing: 801 - 820 of 151085 results

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pGGDestSC+ATG

Plasmid

#49323

Purpose

Destination vector for Golden Gate assembly; Few restriction sites for subcloning.

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-Desmin-N-18

Plasmid

#54060

Purpose

Localization: Intermediate Filaments, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Desmin (DES Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDestattB_ubi:EGFP (pCM272)

Plasmid

#68339

Purpose

Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.

Depositor

Leonard Zon, Christian Mosimann

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Insert

EGFP

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

ubi

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST-ORF-V2

Plasmid

#73638

Purpose

Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Use

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2D3

Plasmid

#15784

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D3 (UBE2D3 Human)

Use

Tags

His

Expression

Bacterial

Mutation

see Depositor Comments below

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pDEST-APLO

Plasmid

#112805

Purpose

gateway cloning destination vector to express LexAop2 driven transgene in Drosophila

Depositor

Chun Han

Article

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2

Plasmid

#149517

Purpose

N-termial APEX2 tag. Gateway destination vector for mammanlian expression

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Type

Empty backbone

Use

Tags

FLAG-APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2R2

Plasmid

#15806

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:miRFP670-cetn4

Plasmid

#192357

Purpose

zfCETN4 tagged with miRFP670, expressed under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

CETN4 (cetn4 Zebrafish)

Use

Tags

miRFP670

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2E1

Plasmid

#15787

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E1 (UBE2E1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pDEST-ORF-V1

Plasmid

#73637

Purpose

Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Use

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2T

Plasmid

#15808

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2T (UBE2T Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pDestTol2-QUAS:GCaMP7a

Plasmid

#184817

Purpose

Used to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GCaMP7a-pA

Use

Zebrafish tol2 transgenesis

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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mEmerald-Desmoglein1-C-18

Plasmid

#54061

Purpose

Localization: Desmosomes, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Desmoglein 1 (DSG1 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST26-MSRA-C-HA

Plasmid

#195177

Purpose

Vector for transient expression of MSRA gene with C-terminal HA tag, gene flanked by att sites for gateway cloning

Depositor

Stephanie Roessler

Insert

MSRA (MSRA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-9xGRE

Plasmid

#74736

Purpose

GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

Depositor

Luisa Dalla Valle

Article

Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

Insert

EGFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

nine GC-responsive elements (9xGRE) from the rat …

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDest-544

Plasmid

#11519

Depositor

Dominic Esposito

Type

Empty backbone

Use

Gateway destination vector

Tags

His6-NusA

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2E2

Plasmid

#15788

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E2 (UBE2E2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pDest27-mBIN1+13+17

Plasmid

#61097

Purpose

Express N-terminal GST tagged-mouse BIN1+13+17 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestGFP_HuD

Plasmid

#65763

Purpose

expresses GFP-tagged HuD in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL4 (ELAVL4 Human)

Use

Tags

FLAG HA and V5

Expression

Mammalian

Mutation

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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