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AAV-hGFAP-NEUROD1-T2A-mCherry

Plasmid

#178582

Purpose

Expression of NEUROD1 and mCherry under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

NEUROD1 (NEUROD1 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-anti-mCherry(LaM6) PAGER(Gq)

Plasmid

#230016

Purpose

Lentiviral vector expressing anti-mCherry PAGER(Gq) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1D

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-anti-mCherry(LaM6) PAGER(Gs)

Plasmid

#230017

Purpose

Lentiviral vector expressing anti-mCherry PAGER(Gs) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1Dxs

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRDAX-483 GFP[1] L9 Stuffer/Intein3 L10 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130166

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-484 GFP[1] L9 Stuffer/Intein4 L10 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130167

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-482 GFP[1] L9 Stuffer/Intein2 L10 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130165

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-481 GFP[1] L9 Stuffer/Intein1 L10 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130164

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe4_ChR2_mCherry

Plasmid

#213945

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe10_ChR2_mCherry

Plasmid

#213815

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe4_ChR2_mCherry

Plasmid

#213937

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe3_ChR2_mCherry

Plasmid

#213941

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer. Alias: AAV_WDP0003_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiCHATe27_ChR2_mCherry

Plasmid

#213830

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiVIPe4_ChR2_mCherry

Plasmid

#213856

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiLAMP5e3_ChR2_mCherry

Plasmid

#213915

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B

Plasmid

#64221

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry-P2A-E1B

Expression

Mammalian

Mutation

Promoter

CBh and U6

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-H2B-PAmCherry

Plasmid

#133419

Purpose

Fluorescent reporter for calcium imaging and photoactivation

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-H2B-PAmCherry (H2BC11 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-DiO-eGFP-2A-mCherry_D4H

Plasmid

#194880

Purpose

Ratiometric D4H sensor for in vivo cre-dependent neuron-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-D4H

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mCherry-3XNLS-WPRE

Plasmid

#159951

Purpose

nucleus marker

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mCherry-3XNLS

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.mCherry-P2A-Post-SPHERE-SF-iGluSnFR

Plasmid

#187102

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Post-SPHERE-SF-iGluSnFR

Use

AAV

Tags

mCherry-P2A

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-DiO-eGFP-2A-mCherry_YDA

Plasmid

#194881

Purpose

Ratiometric YDA sensor for in vivo cre-dependent neuron-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-YDA

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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