We narrowed to 6,501 results for: nls

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pYPQ-NF-L2-dMb2Cas12a

Plasmid

#177684

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Publication

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dMb2Cas12a

Use

CRISPR; Gateway compatible foki-dmb2cas12a entry …

Tags

NLS

Expression

Plant

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSYC-104

Plasmid

#74792

Purpose

pCS4+-NLS-EGFP-F2A-mCherry-CAAX

Depositor

Seok-Yong Choi

Publication

Kim et al PLoS One. 2011 . 6(4):e18556.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLS-EGFP-F2A-mCherry-CAAX

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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yWL234 MCP-GFP-PABPC1*

Plasmid

#197054

Purpose

pUBC-NLS-HA-2xMCP-1xGFP-PABPC1*

Depositor

Robert Singer

Publication

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-GFP-PABPC1*

Use

Lentiviral

Expression

Mammalian

Promoter

ubc

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

Plasmid

#64112

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pMH186

Plasmid

#122837

Purpose

MS2-NLS (no stop codon) with GreenGate C-D flanking sequences

Depositor

Markus Schmid

Publication

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

MS2-NLS, codon-optimised for Arabidopsis

Use

Golden gate compatible cloning vector

Tags

Nuclear localisation signal

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pPS1891

Plasmid

#1363

Depositor

Pamela Silver

Publication

Damelin et al Mol Cell 2000 Jan;5(1):133-40.

Insert

NLS-EYFP

Expression

Yeast

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-eGFP-P2A-LgBiT-NLS

Plasmid

#187834

Purpose

Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

Depositor

William McEwan

Publication

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

eGFP-P2A-LgBiT-NLS

Use

AAV

Promoter

hSyn

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-mCherry-NLS

Plasmid

#165442

Purpose

Expresses nuclear mCherry from CMV promoter

Depositor

Esteban Engel

Publication

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

mCherry-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQCXIP-NLS-Vx3-mEGFP

Plasmid

#35529

Depositor

Robert Cohen

Publication

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

NLS-Vx3

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro

Plasmid

#228399

Purpose

Encodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal PKI NES that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Publication

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

3xFLAG-NLS/SV40-AcGFP-NES/PKI

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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PSD95-TS:YFP

Plasmid

#42225

Depositor

Roger Tsien

Publication

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PSD95

Tags

TS:YFP

Expression

Mammalian

Available since

Feb. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170816

Purpose

Lentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Publication

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mScarlet-I-P2A-BlastR

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Publication

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBK-YE2-BE3

Plasmid

#85176

Purpose

Expresses YE2-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YEE-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pQC NLS mPlum IX

Plasmid

#37356

Depositor

Connie Cepko

Publication

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLS mPlum

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pCL66-decoder

Plasmid

#159453

Purpose

Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.

Depositor

Joerg Stelling

Publication

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pfus1mut-tetR-nls-malE-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfus1mut

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pPS1890

Plasmid

#1362

Depositor

Pamela Silver

Publication

Damelin et al Mol Cell 2000 Jan;5(1):133-40.

Insert

NLS-ECFP

Expression

Yeast

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pEG302 22aa SunTag VP64 nog

Plasmid

#120251

Purpose

CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

Depositor

Steven Jacobsen

Publication

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pTriEx4-Cdc42-2G

Plasmid

#68814

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

Depositor

Olivier Pertz

Publication

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cdc42 activation reporter

Tags

His and Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR

Plasmid

#121836

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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