We narrowed to 5,093 results for: puc

1. pUC19_msfGFP-TCOF1-repair-template

   Plasmid

   #237632

   Purpose

   For knock-in msfGFP to TCOF1 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   TCOF1 (TCOF1 Human)
   

   Use

   CRISPR

   Tags

   msfGFP

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19_msfGFP-FUS-repair-tempate

   Plasmid

   #237684

   Purpose

   For knock-in msfGFP to FUS locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   FUS (FUS Human)
   

   Use

   CRISPR

   Tags

   msfGFP

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19::ZmUBI-RWT4Ca+C-Tail

   Plasmid

   #237514

   Purpose

   Protoplast expression

   Depositor

   Gitta Coaker

   Article

   Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

   Insert

   Rwt4Ca+C-Tail
   

   Expression

   Plant

   Mutation

   Add C-terminal extension

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCmini-RELA-PGK-EGFP

   Plasmid

   #228051

   Purpose

   Homologous donor template for RELA knockout expressing EGFP

   Depositor

   Yohei Sato

   Article

   Sato et al Clinical Immunology Communications, Volu

   Insert

   EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCmini-RELB-PGK-EGFP

   Plasmid

   #228052

   Purpose

   Homologous donor template for RELB knockout expressing EGFP

   Depositor

   Yohei Sato

   Article

   Sato et al Clinical Immunology Communications, Volu

   Insert

   EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUCmini-RELC-PGK-EGFP

   Plasmid

   #228053

   Purpose

   Homologous donor template for RELC knockout expressing EGFP

   Depositor

   Yohei Sato

   Article

   Sato et al Clinical Immunology Communications, Volu

   Insert

   EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUCmini-RELA-PGK-tdTomato

   Plasmid

   #228054

   Purpose

   Homologous donor template for RELA knockout expressing tdTomato

   Depositor

   Yohei Sato

   Article

   Sato et al Clinical Immunology Communications, Volu

   Insert

   tdTomato
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUCmini-RELC-PGK-tdTomato

   Plasmid

   #228056

   Purpose

   Homologous donor template for RELC knockout expressing tdTomato

   Depositor

   Yohei Sato

   Article

   Sato et al Clinical Immunology Communications, Volu

   Insert

   tdTomato
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-hU6-2O-crScaffold_SV40-BFP

   Plasmid

   #224861

   Purpose

   crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from SV40 promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC-hU6-2O-crScaffold_EF1a-BFP

    Plasmid

    #224862

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC-hU6-2O-crBFP_EF1a-BFP

    Plasmid

    #224783

    Purpose

    BFP-targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC-hU6-2O-crEGFP_EF1a-BFP

    Plasmid

    #224784

    Purpose

    EGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crEGFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC-hU6-crBFP_EF1a-BFP

    Plasmid

    #224785

    Purpose

    BFP targeting crRNA for RfxCas13d expressed from hU6 promoter and target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6 and hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC-hU6-CrEGFP_EF1a-BFP

    Plasmid

    #224786

    Purpose

    EGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6 and hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57 mini-FLAG-HA-Regnase-3 WT

    Plasmid

    #222651

    Purpose

    For in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c (Zc3h12c Mouse)
    

    Use

    In vitro transcription

    Tags

    FLAG and HA

    Promoter

    T7

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCmini-NFKB1-PGK-EGFP

    Plasmid

    #204018

    Purpose

    Homologous donor template for NFKB1 knockout expressing EGFP

    Depositor

    Yohei Sato

    Article

    Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

    Insert

    EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCmini-NFKB1-PGK-tdTomato

    Plasmid

    #204019

    Purpose

    Homologous donor template for NFKB1 knockout expressing tdTomato

    Depositor

    Yohei Sato

    Article

    Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

    Insert

    tdTomato
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUCmini-NFKB2-PGK-EGFP

    Plasmid

    #204020

    Purpose

    Homologous donor template for NFKB2 knockout expressing EGFP

    Depositor

    Yohei Sato

    Article

    Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

    Insert

    EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUCmini-NFKB2-PGK-tdTomato

    Plasmid

    #204021

    Purpose

    Homologous donor template for NFKB2 knockout expressing tdTomato

    Depositor

    Yohei Sato

    Article

    Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

    Insert

    tdTomato
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC-T7-hTP53-P0~670-FLuc

    Plasmid

    #208640

    Purpose

    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.

    Depositor

    Quanfu Ma

    Insert

    TP53, UTR and partial CDS (TP53 Human)
    

    Use

    Luciferase; In vitor transcription template

    Tags

    Firefly Luciferase (F-Luc) and T7 promoter

    Mutation

    UTR and partial CDS in-frame with F-Luc

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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