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Showing: 801 - 820 of 2972 results

  1. Epac-S-H189

    Plasmid
    #170350
    Purpose
    mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170350-plasmid-map-sequence-id-336138

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  8. pET22b-T5-sfGFP*-XcP4H-MH4R

    Plasmid
    #178043
    Purpose
    Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP
    Depositor
    Han Xiao
    Publication
    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1
    Inserts
    superfolder green fluorescent protein with TAG at 151 position
    XcP4H with W179F mutation
    dihydropterine reductase from human
    pterin-4a-carbinolamine dehydratase from human
    Tags
    His tag
    Expression
    Bacterial
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    178043-plasmid-map-sequence-id-359586

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  9. pEGFPC1-ORAI1-EC-HA

    Plasmid
    #174335
    Purpose
    encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).
    Depositor
    Sonal Srikanth
    Publication
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human Orai1 (ORAI1 Human)
    Tags
    GFP in the N-terminus and HA tag in the extracell…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174335-plasmid-map-sequence-id-349874

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  10. pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP

    Plasmid
    #50834
    Purpose
    An AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.
    Depositor
    Brandon Harvey
    Publication
    McDevitt et al Cell Rep. 2014 Sep 25;8(6):1857-1869. do
    Insert
    hChR2 (H134R)
    Use
    AAV and Cre/Lox
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    EF1a
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50834-plasmid-map-sequence-id-76270

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  15. NmCas9_sgRNA_expression_in_pBluescript

    Plasmid
    #122091
    Purpose
    U6 driven NmCas9 sgRNA expression vector for cloning own guides
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122091-plasmid-map-sequence-id-242297

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  16. SpCas9_sgRNA_expression_in_pBluescript

    Plasmid
    #122089
    Purpose
    U6 driven SpCas9 sgRNA expression vector for cloning own guides
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122089-plasmid-map-sequence-id-242295

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  17. pYZ033

    Plasmid
    #98404
    Purpose
    Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1
    Depositor
    Jef Boeke
    Publication
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98404-plasmid-map-sequence-id-198860

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  18. BcLOV4 fusion tools screening plasmid #2

    Plasmid
    #174512
    Purpose
    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.
    Depositor
    Brian Chow
    Publication
    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
    Tags
    3xFLAG and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    174512-plasmid-map-sequence-id-357202

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Showing: 801 - 820 of 2972 results