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pAcBac3-EcTrp-TGA-EGFP**

Plasmid

#228786

Purpose

EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppression

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTrpRS
EcWtR
EGFP**

Use

Baculoviral

Tags

Expression

Mammalian

Mutation

TGA suppressor

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223 ATG3 C264A

Plasmid

#129293

Purpose

Gateway entry clone encoding human ATG3 C264A (catalytic inactive)

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

changed Cysteine 264 to Alanine

Promoter

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 ATG3 allKR

Plasmid

#129295

Purpose

Gateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginine

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

changed all 22 Lysine residues to Arginine

Promoter

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B Q116P G120

Plasmid

#129291

Purpose

Gateway entry clone encoding human deconjugation-resistant LC3B

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Changed Glutamine 116 to Proline. Deleted amino a…

Promoter

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-nAChr-Alpha6-GFP

Plasmid

#50486

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr-alpha6 (Chrna6 Mouse)

Use

Tags

GFP fusion in M3-M4 loop (after residue A405)

Expression

Mammalian

Mutation

Gly-Ala-Gly flexible linker flanking the GFP open…

Promoter

CMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-nAChr-beta3-YFP

Plasmid

#50484

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

YFP fusion in M3-M4 loop (after residue P379). O…

Promoter

CMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-nAChr-Alpha6-YFP

Plasmid

#50483

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr-alpha6 (Chrna6 Mouse)

Use

Tags

YFP fusion in M3-M4 loop (after residue A405)

Expression

Mammalian

Mutation

Gly-Ala-Gly flexible linker flanking the YFP open…

Promoter

CMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-nAChr-beta3-CFP

Plasmid

#50485

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP fusion in M3-M4 loop (after residue P379). O…

Promoter

CMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-nAChr-Alpha6-CFP

Plasmid

#50482

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr-alpha6 (Chrna6 Mouse)

Use

Tags

CFP fusion in M3-M4 loop (after residue A405)

Expression

Mammalian

Mutation

Gly-Ala-Gly flexible linker flanking the CFP open…

Promoter

CMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pJBL7055

Plasmid

#167224

Purpose

orthogonal T7 RNAP sfGFP reporter

Depositor

Julius Lucks

Insert

pRV-sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL7057

Plasmid

#167226

Purpose

orthogonal T7 RNAP sfGFP reporter

Depositor

Julius Lucks

Insert

pIRH-sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL7058

Plasmid

#167227

Purpose

overexpression of orthogonal T7 RNAP

Depositor

Julius Lucks

Insert

J23119-AKSIRV

Use

Synthetic Biology

Tags

6X His

Expression

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pEN_TT CVB3 A67G

Plasmid

#216788

Purpose

Gateway entry vector for the Kandolf strain of CVB3 with a VP4 A67G mutation

Depositor

Kevin Janes

Insert

CVB3

Use

Entry vector for gateway cloning

Tags

Expression

Mutation

A67G in VP4

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7065

Plasmid

#167234

Purpose

cell-free cascaded nitrate sensor

Depositor

Julius Lucks

Insert

pNar-AKSIRV

Use

Synthetic Biology

Tags

6X His

Expression

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL7066

Plasmid

#167235

Purpose

cell-free cascaded mercury sensor

Depositor

Julius Lucks

Insert

pMer-AKSIRV

Use

Synthetic Biology

Tags

6X His

Expression

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL7069

Plasmid

#167238

Purpose

malachite green aptamer tagged sfGFP

Depositor

Julius Lucks

Insert

J23119-sfGFP-MGA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL7070

Plasmid

#167239

Purpose

malachite green aptamer tagged sfGFP

Depositor

Julius Lucks

Insert

pT7-sfGFP-MGA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL7090

Plasmid

#167252

Purpose

cell-free mercury sensor with enzymatic output

Depositor

Julius Lucks

Insert

pMer-XylE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL7091

Plasmid

#167253

Purpose

cell-free arsenic sensor with enzymatic output

Depositor

Julius Lucks

Insert

pArs-XylE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL7093

Plasmid

#167254

Purpose

cell-free lead sensor with XylE output

Depositor

Julius Lucks

Insert

pPbr-XylE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge