We narrowed to 9,520 results for: try

1. pCI-Flag-NEDD4L∆WW1,2,4

   Plasmid

   #171137

   Purpose

   expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L (NEDD4L Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed tryptophan 221 to alanine, tryptophan 453…

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223 LC3B no-stop

   Plasmid

   #123205

   Purpose

   Gateway entry clone encoding human MAP1LC3B lacking stop codon, suitable for C-terminal tagging

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Stop codon removed

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223 LC3A no-stop

   Plasmid

   #123204

   Purpose

   Gateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal tagging

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Stop codon removed

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223 GABARAPL2 G116A

   Plasmid

   #123087

   Purpose

   Gateway entry clone encoding human GABARAPL2 G116A

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Glycine 116 to Alanine

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR E2 HSPter-RPS5Ap-mito

   Plasmid

   #163146

   Purpose

   Plant mitoTALEN vector assembly, Entry clone 2 with attL1 L4; Multisite Gateway entry vector to terminate the E1 ORF and having RPS5A promoter with mitochondrial presequence for the E3 ORF

   Depositor

   Shin-ichi Arimura

   Article

   Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

   Insert

   A terminator of Arabidopsis heat shock protein, RPS5A promoter, and mitochondrial presequence
   

   Use

   Entry vector

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223 GABARAPL2 G116

   Plasmid

   #123088

   Purpose

   Gateway entry clone encoding human GABARAPL2 G116

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Deleted amino acid 117. Stop codon after G116

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223 LC3A G120

   Plasmid

   #123071

   Purpose

   Gateway entry clone encoding human MAP1LC3A G120

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Deleted amino acid 121. Stop codon after G120.

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223 LC3B G120

   Plasmid

   #123074

   Purpose

   Gateway entry clone encoding human MAP1LC3B G120

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Deleted amino acids 121-125. Stop codon after G12…

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223 GABARAPL1 G116

   Plasmid

   #123085

   Purpose

   Gateway entry clone encoding human GABARAPL1 G116

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Deleted amino acid 117. Stop codon after G116

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTMiniTrex-mCherry-Shark3

    Plasmid

    #233145

    Purpose

    Serves as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassette

    Depositor

    Ronald Etheridge

    Article

    Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

    Inserts

    mCherry
    Shark3
    

    Use

    Prokaryotic expression

    Tags

    3x Ty tag

    Mutation

    Codon optimized for Trypanosoma cruzi and changed…

    Available Since

    March 20, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pDONR223 GABARAPL1 no-stop

    Plasmid

    #123210

    Purpose

    Gateway entry clone encoding human GABARAPL1 lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Stop codon removed

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223 GABARAPL2 no-stop

    Plasmid

    #123211

    Purpose

    Gateway entry clone encoding human GABARAPL2 lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Stop codon removed

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTMiniTrex-mCherry-Shark1

    Plasmid

    #233143

    Purpose

    Serves as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassette

    Depositor

    Ronald Etheridge

    Article

    Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

    Inserts

    mCherry
    Shark1
    

    Use

    Prokaryotic expression

    Tags

    3x Ty tag

    Mutation

    Codon optimized for Trypanosoma cruzi and changed…

    Available Since

    March 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAcBac1-EcTrp-TGA

    Plasmid

    #228780

    Purpose

    aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTrpRS
    EcWtR
    

    Use

    Baculoviral

    Expression

    Mammalian

    Mutation

    TGA suppressor

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-nAChr-Alpha6

    Plasmid

    #48812

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3.1-nAChr-beta3

    Plasmid

    #48811

    Purpose

    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

    Depositor

    Jerry Stitzel, Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223 ATG3 WT

    Plasmid

    #129292

    Purpose

    Gateway entry clone encoding wild-type human ATG3 with a stop codon

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Gateway entry vector / entry clone

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223 ATG3 K243R

    Plasmid

    #129294

    Purpose

    Gateway entry clone encoding human ATG3 K243R

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    changed Lysine 243 to Arginine

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223 LC3B G120A no-stop

    Plasmid

    #123206

    Purpose

    Gateway entry clone encoding human MAP1LC3B G120A lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Glycine 120 to Alanine, stop codon removed

    Available Since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cas9/pTREX-n

    Plasmid

    #68708

    Purpose

    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Insert

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    

    Use

    CRISPR

    Tags

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

    Mutation

    C4239T mutation that eliminates a BamHI restricti…

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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