We narrowed to 17,594 results for: cript

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GAL4-BRD4

Plasmid

#21943

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag and GAL4-DNA-BD

Expression

Bacterial

Mutation

aa 1209-1362

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
pH6HTC_IGF2BP1-Halo

Plasmid

#175300

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP1 (IGF2BP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAdv/TERT

Plasmid

#26744

Depositor

Bingliang Fang

Article

Gu et al Cancer Res. 2000 Oct 1. 60(19):5359-64.

Insert

human TERT promoter (TERT Human)

Expression

Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Plasmid

#58439

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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QUAS-5-T7-TetO-GFP_ColE1

Plasmid

#171660

Purpose

QUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-5-T7-TetO-GFP ssra-T7 Stop

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPPC005

Plasmid

#171140

Purpose

For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

XylS-Pm

Use

CRISPR

Expression

Bacterial

Promoter

Pm

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116955

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-FOXR2

Plasmid

#153151

Purpose

Expression of Flag tagged human FOXR2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXR2 (FOXR2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-92a

Plasmid

#22767

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 92a (mir-92a primary transcript, Fly)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200)

Plasmid

#119857

Purpose

Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) (YTHDF1 100-200 subdomain)

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-NYTHDF1(100-200)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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SP6-hCas9-Ce-mRNA

Plasmid

#47911

Purpose

In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.

Depositor

Paul Sternberg

Article

Chiu et al Genetics. 2013 Aug 26.

Insert

hCas9 with C. elegans 5' and 3' UTRs

Use

CRISPR

Promoter

SP6

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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SNAPtag-LacI

Plasmid

#183922

Purpose

Lac repressor labeled with SNAPtag; can be used to label lacO sites

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

SNAPtag-LacI (lacI Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_IRF3

Plasmid

#187749

Purpose

MAC-tagged gene expression of human IRF3

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

IRF3 (IRF3 Human)

Expression

Mammalian

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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YFP-Sp100C(W655Q)-C3

Plasmid

#134556

Purpose

Expresses YFP-Sp100C fusion protein with a W655Q mutation

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2013 Mar 13.

Insert

Sp100C(W655Q)

Tags

YFP

Expression

Mammalian

Mutation

W655Q point mutation in the SAND domain

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRN3P_T3_BE4_IVT

Plasmid

#171762

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

Depositor

Timo Otonkoski

Article

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Insert

BE4

Use

Vector for in-vitro transcription

Promoter

T3 promoter

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_MANF-Halo

Plasmid

#175341

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

MANF (MANF Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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KBB418

Plasmid

#185088

Purpose

Swi6L-GFP with nuclear export signal mutated to alanines

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

Swi6 nuclear export signal L to A mutations

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pNC1011

Plasmid

#42898

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

3 tandem copies of GFP*

Use

Yeast integrating

Mutation

changed Phenylalanine 64 to Leucine, Serine 65 t…

Promoter

None

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO1_F

Plasmid

#146314

Purpose

Mammalian Expression of HsAGO1

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO1 (AGO1 Human)

Expression

Mammalian

Mutation

N574S

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HNRNPC-Halo

Plasmid

#175306

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HNRNPC (HNRNPC Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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