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pcDNA3-HA-RAPGEF2

Plasmid

#110162

Purpose

Expression of human RAPGEF2 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

Insert

RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-BFP

Plasmid

#107722

Purpose

For expression of sgRNA from the U6 promoter with BFP expression simultaneously

Depositor

Xingxu Huang

Article

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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iRFP-Sec61beta

Plasmid

#108125

Purpose

ER marker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

iRFP:SEC61B (SEC61B Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3 1 kb prom. + Enhancer CD274

Plasmid

#107005

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Mutation

N.B. There are two published SNPs in this region …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hygro(+)-PSTCD-cGAS

Plasmid

#102605

Purpose

Mammalian expression of cGAS fused to PSTCD tag

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

cGAS (CGAS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-GST-4EBP1-Low GC

Plasmid

#69018

Purpose

expresses tagged 4EBP1

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

EIF4EBP1 (EIF4EBP1 Human)

Tags

GST and HA

Expression

Mammalian

Mutation

lowGC content, wt is ~70% GC rich. Still, it code…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-mFgf21pro1-luc

Plasmid

#101797

Purpose

Reporter for mouse Fgf21 promoter activity

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

Fibroblast growth factor 21 promoter (Fgf21 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pNat-AID*-9myc

Plasmid

#99523

Purpose

C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+ H2B-mCherry

Plasmid

#99265

Purpose

Contains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

Depositor

Philipp Keller

Article

Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

Insert

mCherry

Tags

H2B

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-mCherry-kanMX6

Plasmid

#89068

Purpose

genomic GBP-mCherry epitope C-terminal tagging in S. pombe

Depositor

Quanwen Jin

Article

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

GBP-mCherry

Expression

Yeast

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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ITPKA-mScarlet-I

Plasmid

#98829

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

ITPKA

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgp53/Cre

Plasmid

#89646

Purpose

Expresses a p53-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting p53 (Trp53 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMonAID_nCas9-PmCDA_Hyg_ALS

Plasmid

#91693

Purpose

Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

2x35S

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-VCre

Plasmid

#89575

Purpose

Expresses VCre under pCAG

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

VCre recombinase

Use

Cre/Lox and Synthetic Biology

Tags

NLS

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAP1_WT_V5

Plasmid

#82994

Purpose

Gateway Donor vector containing TRAP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

TRAP1 (TRAP1 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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LOTUS-V/pcDNA3

Plasmid

#87127

Purpose

Bioluminescent and ratiometric voltage indicator

Depositor

Takeharu Nagai

Article

Inagaki et al Sci Rep. 2017 Feb 13;7:42398. doi: 10.10

Insert

LOTUS-V

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

Plasmid

#86413

Purpose

nuclear split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

Nop1pr-GFP11-mCherry-PUS1

Tags

GFP11 and mCherry

Expression

Yeast

Promoter

NOP1

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSLIK DN RhoA

Plasmid

#84646

Purpose

Lentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of Venus

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

RhoA (RHOA Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Dominant Negative

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only
pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

Plasmid

#84247

Purpose

Expresses direct fusion VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

Plasmid

#67975

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AZsG cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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