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Jag1ICD-HA pBacPAK8-3

Plasmid

#17331

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Jagged1 (Jag1 Rat)

Tags

HA

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pFastBac1-OSF-TRIM5alpha(human)(R332P)

Plasmid

#79037

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human R332P) (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Arg 332 to Pro

Promoter

polyhedrin

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCaSpeR4 cyto Grx1-roGFP2

Plasmid

#64999

Purpose

Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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DYNLL1

Plasmid

#132530

Purpose

Human LC8-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLL1 (DYNLL1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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DYNC2LI1

Plasmid

#132527

Purpose

Human LIC3, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNC2LI1 (DYNC2LI1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLRB1

Plasmid

#132533

Purpose

Human Rbl-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLRB1 (DYNLRB1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pUASp-FMW-attB-AthHen1

Plasmid

#104957

Purpose

Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila germline tissues using Gal4 drivers

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Tags

FLAG Myc

Expression

Insect

Promoter

pTrasposase promoter

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(K14A)

Plasmid

#86562

Purpose

Expresses human full-length Akt1(K14A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

K14A mutation in the PH domain

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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hs-lambda btl

Plasmid

#17581

Depositor

Denise Montell

Article

Lee et al Genes Dev. 1996 Nov 15. 10(22):2912-21.

Insert

lambda-btl (btl Fly)

Expression

Insect

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pBacPAK8-His-myc-Uba1

Plasmid

#29508

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Uba1 (UBA1 Budding Yeast)

Tags

His and myc

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159673

Purpose

Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Tags

T2A-GFP

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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Stat92e pPAcPL

Plasmid

#8713

Depositor

Jim Darnell

Article

Yan et al Cell 1996 Feb 9;84(3):421-30.

Insert

Stat92e (Stat92E Fly)

Expression

Insect

Available Since

July 20, 2005

Availability

Academic Institutions and Nonprofits only
AAEL007584-Cas9

Plasmid

#100706

Purpose

The promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL007584-hspcas9-GFP
Opie2-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL007584 and Opie2

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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Djun - sense

Plasmid

#38337

Depositor

Norbert Perrimon

Article

Zhang et al Proc Natl Acad Sci U S A. 1990 Aug;87(16

Insert

djun (Jra Fly)

Expression

Insect

Promoter

Hsp70

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pVMG0173_Cq-Actin5c-Cas9

Plasmid

#169345

Purpose

Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0173_Cq-Actin5c-Cas9

Use

CRISPR

Expression

Insect

Promoter

CPIJ009808

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-luc-CLK

Plasmid

#33132

Depositor

Michael Rosbash

Article

Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. E

Insert

CLK 3' UTR (Clk Fly)

Use

Luciferase

Tags

Firefly Luciferase

Expression

Insect

Promoter

Actin 5C

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pVMG0212_Cq-nanos-Cas9

Plasmid

#169348

Purpose

Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0212_Cq-nanos-Cas9

Use

CRISPR

Expression

Insect

Promoter

CPIJ011551

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pVMG0213_Cq-vasa-Cas9

Plasmid

#169347

Purpose

Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0213_Cq-vasa-Cas9

Use

CRISPR

Expression

Insect

Promoter

CPIJ009286

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-His6-Mps1-KD

Plasmid

#114064

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

Insert

Mps1-KD (TTK Human)

Tags

His6

Expression

Insect

Mutation

Mps1 KD (D664A)

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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UAS-u(XL)Cas9

Plasmid

#127386

Purpose

Expresses Cas9 at very low level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(XL)-Cas9

Use

CRISPR

Expression

Insect

Promoter

UAST

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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