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Atp5-DHFR-TEV (integrating)

Plasmid

#247219

Purpose

Plasmid for genomic integration of Atp5 (MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.

Depositor

Johannes Herrmann

Publication

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

Atp5 MTS-DHFR-TEV

Expression

Yeast

Promoter

TEF2

Available since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Oxa1-DHFR-TEV (integrating)

Plasmid

#247220

Purpose

Plasmid for genomic integration of Oxa1(MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.

Depositor

Johannes Herrmann

Publication

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

Oxa1 MTS-DHFR-TEV

Expression

Yeast

Promoter

TEF2

Available since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (Beta)

Plasmid

#241109

Purpose

Expresses SARS-CoV-2 spike (Beta) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (Beta) (S SARS-CoV-2)

Expression

Mammalian

Mutation

L18F,D80A,D215G,L241del,L242del,A243del,K417N,E48…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pA-CBh.TP-CNOT7-V5H6.MCh.Puro

Plasmid

#209946

Purpose

In mammalian cells expresses TP fused to a CNOT7 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

Depositor

Kevin Weeks

Publication

Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

Inserts

CCR4-NOT transcription complex subunit 7 (CNOT7 Human)
mCherry fluorescent protein fused to puromycin resistance marker

Tags

TP (MS2 coat protein) and V5 epitope with H6 tag

Expression

Mammalian

Promoter

CBh and CMV

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_MSCV-NUP98::LNP1-ires-GFP

Plasmid

#237494

Purpose

Express NUP98::LNP1 in bicistronic retroviral vector

Depositor

Charles Mullighan

Publication

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

NUP98::LNP1 (LNP1, NUP98 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA -CLCN5-3xFlag

Plasmid

#225507

Purpose

CLCN5 expression vector

Depositor

Wade Harper

Publication

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CLCN5 (CLCN5 Human)

Tags

3xFlag

Expression

Mammalian

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 Flag-LplA

Plasmid

#232118

Purpose

For bacterial expression of Flag-tagged E. coli LplA.

Depositor

Ruth Sommese

Publication

Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

Insert

lplA (lplA Escherichia coli (strain K12))

Tags

Flag

Expression

Bacterial

Promoter

T7 Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-LNI (with mNgn2x3HA)

Plasmid

#233195

Purpose

Retroviral expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming

Depositor

Kate Galloway

Publication

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mLhx3-P2A-mNgn2x3HA-T2A-Isl1 (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pC131: pAAV.CMV-CasRx-VEGFA sgRNA array

Plasmid

#203444

Purpose

Plasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAs

Depositor

Guei-Sheung Liu

Publication

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA array
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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WT USP7 TRAF

Plasmid

#227226

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

aa 62-205

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AAVS1_dCas9-XTEN-KRAB-p2A-GFP

Plasmid

#222108

Purpose

Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

Depositor

Jonathan Weissman

Publication

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)

Use

AAV and CRISPR

Tags

HA and P2A-GFP

Expression

Mammalian

Mutation

D10A, H840A

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-NES-jRGECO1a-WPRE

Plasmid

#216276

Purpose

AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1a

Depositor

Guohong Cui

Publication

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

NES-jRGECO1a

Use

AAV and Cre/Lox

Tags

6XHIS and Xpress

Promoter

human synapsin 1 (hSyn)

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

Plasmid

#202059

Purpose

Inducible AAV activator

Depositor

Michael Bassik, Lacra Bintu

Publication

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

Use

AAV; Aav with reporter

Expression

Mammalian

Mutation

ITRs intact

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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MEF2A-Sensor

Plasmid

#208928

Purpose

in vitro MAPK-Sensor for luminescent detection of ERK and p38-activity

Depositor

Attila Reményi

Publication

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

Mef2a docking sequence+Phospho site (MEF2A Human)

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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DNAJC11-EGFP

Plasmid

#210368

Purpose

Expresses DNAJC11 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Publication

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DNAJC11 (DNAJC11 Human)

Tags

EGFP

Expression

Mammalian

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

Plasmid

#172984

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Publication

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (1-170)

Plasmid

#203426

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Publication

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (1-208)

Plasmid

#203427

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Publication

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (1-277)

Plasmid

#203428

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Publication

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL delta PBM + FBM

Plasmid

#202416

Purpose

Expression of GFP-tagged PODXL PDZ-binding and FERM-binding motif mutation

Depositor

David Bryant

Publication

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Mutation

Lacking DTHL, and H477/R479/S481>AAA mutation

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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