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pMJA065

Plasmid

#128517

Purpose

Expresses Ber e 1 in Pichia

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

Insert

Ber e 1 a allergenic 2S albumin from Brazil nut

Expression

Yeast

Promoter

AOX1

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-FSF1

Plasmid

#110641

Purpose

Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

FSF1 (FSF1 Budding Yeast)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBluescript_VEGFA-TS3_SpCas9

Plasmid

#107328

Purpose

U6 driven SpCas9 sgRNA expression for VEGFA site 3

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

VEGFA-TS3 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBluescript_VEGFA-TS3_NmCas9

Plasmid

#107329

Purpose

U6 driven NmCas9 sgRNA expression for VEGFA site 3

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

VEGFA-TS3 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 D407A

Plasmid

#111795

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D407A

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 R419A

Plasmid

#111792

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R419A

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 D406A

Plasmid

#111794

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D406A

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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r-22. pT7-7-SNCA-9Asn

Plasmid

#107297

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 22 aa C-terminal moiety, which consists of 9 Asn and 13 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-9Asn (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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t2. pT7-7-SNCA-5Lys

Plasmid

#107294

Purpose

Plasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Lys (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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r-6. pT7-7-SNCA-2Asn

Plasmid

#107296

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 6 aa C-terminal moiety, which consists of 2 Asn and 4 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-2Asn (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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r. pT7-7-SNCA-5Asn

Plasmid

#107295

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Asn (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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s2. pT7-7-SNCA-5Asp

Plasmid

#107293

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Asp (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAP1791-1

Plasmid

#105246

Purpose

human eGFP with homology arms for RAB11A (recoded )

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with homology arms for RAB11A (recoded *) (RAB11A Human)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pconst #4-RFP

Plasmid

#81078

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23107

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #3-RFP

Plasmid

#81077

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23101

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pWHI2-NAT

Plasmid

#80588

Purpose

CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

Depositor

John McCusker

Article

Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

Insert

WHI2 (WHI2 Budding Yeast)

Expression

Yeast

Promoter

Native WHI2 promoter

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pCS3-MT-BX/ARF1-64

Plasmid

#78762

Purpose

To overexpress ARF1-64 in Mammalian Cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

ARF (CDKN2A Human)

Tags

6xMYC

Expression

Mammalian

Mutation

1-64

Promoter

CMV IE94

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
pCS3-MT-BX/ARF65-132

Plasmid

#78763

Purpose

To overexpress ARF65-132 in Mammalian Cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

ARF (CDKN2A Human)

Tags

6xMYC

Expression

Mammalian

Mutation

65-132

Promoter

CMV IE94

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
AEB-TMR10pUC19

Plasmid

#63858

Purpose

This pUC19 vector contains a tetramethylrosamine (TMR) binding riboswitch, controlling the translation initaition rate of luciferase gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

TMR10 TMR riboswitch

Use

Synthetic Biology

Promoter

tac

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only
pGL2-HKII-E

Plasmid

#64517

Purpose

AS-30D hepatoma hexokinase II 0.1 kbp promoter-luciferase reporter vector

Depositor

Peter Pedersen

Article

Mathupala et al J Biol Chem. 2001 Nov 16;276(46):43407-1

Insert

Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)

Use

Luciferase

Tags

firefly luciferase

Mutation

Isolated from AS-30D rat hepatoma

Promoter

Hexokinase II

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only