We narrowed to 10,356 results for: plasmids 101

1. pscALPSpuro-RnACE2 (Rat)

   Plasmid

   #158086

   Purpose

   Expresses rat ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Rat ACE2 (Ace2 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-nSpCas9-TadA8e

   Plasmid

   #209044

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TadA8e-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. B2M Bulldozer (gRNA crB2M_13)

   Plasmid

   #84381

   Purpose

   This plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cells

   Depositor

   Chad Cowan

   Article

   Mandal et al Cell Stem Cell. 2014 Nov 6;15(5):643-52.

   Insert

   B2M Bulldozer crB2M_13 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 promoter

   Available Since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pscALPSpuro-FcACE2 (Cat)

   Plasmid

   #158082

   Purpose

   Expresses cat ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Cat ACE2 (ACE2 )
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS

   Plasmid

   #128833

   Purpose

   Knock-in of nuclear EYFP into mouse Elf5 locus

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   Nuclear EYFP into Elf5 locus (Elf5 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

   Plasmid

   #135812

   Purpose

   This plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

   Depositor

   Nadia Mercader Huber

   Article

   Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

   Insert

   TagRFP
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   UAS

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MEK1C222

   Plasmid

   #164638

   Purpose

   Bacterial expression plasmid for His6-MEK1C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   G(-19)F/S222C/C277S/C376S

   Promoter

   T7

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

   Plasmid

   #136652

   Purpose

   Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-hygro-2xMyc-Rac1-Q61L

   Plasmid

   #128582

   Purpose

   For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   2xMyc epitope

   Expression

   Mammalian

   Mutation

   Changed Glutamine61 to Leucine.

   Promoter

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-P3-Alb-Rosmarinus

    Plasmid

    #183462

    Purpose

    To produce AAV with the P3 promoter to express Alb(mouse)-rosmarinus fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    Rosmarinus

    Expression

    Mutation

    Promoter

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-P3-Alb-mCarmine

    Plasmid

    #183464

    Purpose

    To produce AAV with the P3 promoter to express Alb-mCarmine fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    mCarmine

    Expression

    Mutation

    Promoter

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX307_PDHA2S293A

    Plasmid

    #115197

    Purpose

    Lentiviral transduction and expression of PDHA2S293A into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PDHA2S293A (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    p.S293A

    Promoter

    EF1alpha

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP

    Plasmid

    #47905

    Purpose

    An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) under the CaM Kinase IIa promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CaMKIIa

    Available Since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNIC-ZB-SPRTN-E112A-FL

    Plasmid

    #110218

    Purpose

    Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    His6 and ZB

    Expression

    Bacterial

    Mutation

    E112A, P296L (see depositor comments below)

    Promoter

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Syn-SomArchon-mTagBFP2-p2A-CoChR-Kv2.1

    Plasmid

    #206006

    Purpose

    Expression of SomArchon-mTagBFP2 and CoChR under Syn promoter

    Depositor

    Xue Han

    Article

    Lowet et al Cell Rep. 2023 Aug 29;42(8):112906. doi:

    Insert

    SomArchon-mTagBFP2-P2A-CoChR-Kv2.1
    

    Use

    AAV

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ADSS_pLX307

    Plasmid

    #98316

    Purpose

    Lentiviral expression of ADSS

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    ADSS (ADSS2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAP2K3_pLX307

    Plasmid

    #98355

    Purpose

    Lentiviral expression of MAP2K3

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    MAP2K3 (MAP2K3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Q85K

    Promoter

    E1Fa

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. MEK1C218

    Plasmid

    #164639

    Purpose

    Bacterial expression plasmid for His6-MEK1C218

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    G(-19)F/S218C/C277S/C376S

    Promoter

    T7

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. DIMT1_pLX307

    Plasmid

    #98330

    Purpose

    Lentiviral expression of DIMT1

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    DIMT1 (DIMT1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNIC-ZB-SPRTN-Y117C-FL

    Plasmid

    #110219

    Purpose

    Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    His6 and ZB

    Expression

    Bacterial

    Mutation

    Y117C, P296L (see depositor comments below)

    Promoter

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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