We narrowed to 91,394 results for: mal

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p21 promoter E

Plasmid

#16466

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
AAV-SYN-integrin alpha9-V5

Plasmid

#203729

Purpose

AAV vector plasmid expressing human integrin subunit alpha 9 (ITGA9) fused to V5-tag under the human synapsin (SYN) promoter

Depositor

Richard Eva, Bart Nieuwenhuis

Insert

ITGA9 (ITGA9 Human)

Use

AAV

Tags

V5-tag

Expression

Mammalian

Promoter

Human synapsin promoter

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK1(1-828)

Plasmid

#38198

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 829-1051

Available Since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
pHIE055 ZF43x3-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138736

Purpose

Integration Vector, assembled with pHIE047 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE052 ZF43x3-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138733

Purpose

Integration Vector, assembled with pHIE044 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE051 ZF43x1 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138732

Purpose

Integration Vector, assembled with pHIE043 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x1

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE056 ZF43x6-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138737

Purpose

Integration Vector, assembled with pHIE048 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE057 ZF43x12-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138738

Purpose

Integration Vector, assembled with pHIE049 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE054 ZF43x12-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138735

Purpose

Integration Vector, assembled with pHIE046 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf137

Plasmid

#12825

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf137

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

Plasmid

#69227

Purpose

Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_EYFP

Plasmid

#167882

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-fSFRP2-GFP

Plasmid

#22915

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu secreted frizzled receptor protein 2 promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only
pJAF216

Plasmid

#52405

Purpose

Expresses Flag-tagged mouse Arf4

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf4 (Arf4 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pTS1026-Tier1-PhCMV-3xNLS-iRFP670

Plasmid

#169532

Purpose

Tier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nucleus-localized far-red fluorescent protein iRFP670

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTEV5_ RpMatB_4

Plasmid

#78208

Purpose

Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak binding

Depositor

Martin Webb

Article

Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

Insert

matB (His6 C106A R286C T303A Q457C K488A)

Tags

His6

Expression

Bacterial

Mutation

C106A R286C T303A Q457C K488A

Promoter

T7

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTEV5_ RpMatB_3

Plasmid

#78207

Purpose

Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding

Depositor

Martin Webb

Article

Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

Insert

matB (His6 C106A T167A R286C Q457C K488A)

Tags

His6

Expression

Bacterial

Mutation

C106A T167A R286C Q457C K488A

Promoter

T7

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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cycLuc_SbMVs

Plasmid

#119209

Purpose

Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

cyclic luciferase with SbMVp cleavage site

Use

Luciferase

Tags

AU1 tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRger3-TS-EYFP

Plasmid

#127241

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger3-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

CamKIIa

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4A

Plasmid

#115171

Purpose

Expresses membrane-targeted Sema4A with N-terminal myc tag

Depositor

Suzanne Paradis

Article

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

Sema4A (Sema4a Mouse)

Tags

membrane targeting sequence and myc

Expression

Mammalian

Mutation

First 32 amino acids of Sema4A replaced with memb…

Promoter

CMV

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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