We narrowed to 16,506 results for: grna

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pU6_(GLuc)_INT[3xK-T]

Plasmid

#68429

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three kink-turns

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pSpCas9 LP400~3p (pKBA314)

Plasmid

#254039

Purpose

Express SpCas9 and the LP400~3p gRNA

Depositor

Jef Boeke

Article

Barriball et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

gRNA_LP400~3p

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pSpCas9 UGT1+LP400~3p (pNCH0155)

Plasmid

#254042

Purpose

Express SpCas9 and two gRNAs: UGT1 and LP400~3p

Depositor

Jef Boeke

Article

Barriball et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

UGT1 + LP400~3p

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pX330 - LRR1 Terminal CRISPR

Plasmid

#221705

Purpose

Cas9 targeting plasmid with gRNA specific for LRR1 N-terminus

Depositor

Aga Gambus

Article

Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

Insert

TGTAGCTTCATCTCGCCCAA

Use

CRISPR

Promoter

Chicken Beta-actin

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-bleo-ErbB4

Plasmid

#197353

Purpose

A knock-out vector for dog ErbB4.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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E42_gControl_dTet_IRES_mTurquoise2

Plasmid

#189799

Purpose

Retroviral delivery of control guide RNA

Depositor

Ellen Rothenberg

Article

Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

Insert

Luciferase gRNA

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-mGreenLantern/EGFP

Plasmid

#188482

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern/EGFP sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-AtpU6-29

Plasmid

#160219

Purpose

AtpU6-29 Golden Gate level 0 piece to express gRNAs

Depositor

Matias Kirst

Article

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

pAtU6-29 promoter

Use

Golden gate level 0 piece to express grnas

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pgTS40

Plasmid

#169630

Purpose

pX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

U6-driven gRNA expression and PCAG-driven SpCas9 expression

Expression

Mammalian

Promoter

U6 / PCAG

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 Grin1-smFP-V5 KI

Plasmid

#183439

Purpose

FlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP V5 donor

Use

CRISPR

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEDJ-87

Plasmid

#163085

Purpose

MarkerFree plasmid for integration of pADH1-MCP-VPR into site XI-5

Depositor

Michael Jensen

Article

Jensen et al Microb Cell Fact. 2017 Mar 15;16(1):46.

Insert

VPR

Tags

MCP

Expression

Yeast

Promoter

ADH1

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-pleCopyCatcher

Plasmid

#174061

Purpose

CopyCatcher insertion donor for the pale locus

Depositor

Ethan Bier

Article

Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

Insert

Pale (Pale Synthetic, Fly)

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGuide-P1-O1a (EL702)

Plasmid

#140040

Purpose

CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.

Depositor

Johan Elf

Article

Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:

Insert

barcode P1 and sgRNA lacO1 array

Use

Synthetic Biology

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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S1_AscI_V5-gateway_Bsu36I

Plasmid

#128467

Purpose

destination vector with N-terminal V5 tag

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

destination vector with N-terminal V5 tag

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S2_AscI_HA-gateway_Bsu36I

Plasmid

#128468

Purpose

destination vector with N-terminal HA tag

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

destination vector with N-terminal HA tag

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-U61-SapI

Plasmid

#112808

Purpose

gRNA cloning vector for expressing 1-3 gRNAs in Drosophila

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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sgPYM1

Plasmid

#105249

Purpose

human PYM1 sgRNA

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

sgRNA for PYM1

Expression

Mammalian

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only
pPN220

Plasmid

#91681

Purpose

Express sgRNA targeting human VRK2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

VRK2 (VRK2 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN221

Plasmid

#91682

Purpose

Express sgRNA targeting human VRK2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

VRK2 (VRK2 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN154

Plasmid

#91683

Purpose

Express sgRNA targeting human ZNF536

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

ZNF536 (ZNF536 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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