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MARIO/ pcDNA3

Plasmid

#124838

Purpose

mammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensor

Depositor

Takeharu Nagai

Article

Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

Insert

MARIO

Tags

No tag

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-3XNeon-nls

Plasmid

#75386

Purpose

PCP-3XNeon-nls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

PCP

Use

Lentiviral

Tags

3XmNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-CyclinD1(WT)

Plasmid

#122300

Purpose

expresses Cyclin D1 protein with a Myc tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

CCND1 (CCND1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-GST-eIF4E K119A

Plasmid

#112818

Purpose

Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Eif4e (Eif4e Mouse)

Tags

GST

Expression

Bacterial

Mutation

K119 mutated to A, increasing affinity for cap st…

Promoter

T7

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pH-PABE-7-esgRNA

Plasmid

#115620

Purpose

Targeted A to G in rice

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

wtTadA-TadA7.10-nCas9-3*NLS

Use

CRISPR

Expression

Plant

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-BLST-dCas9-VPR

Plasmid

#115148

Purpose

Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

BlastR-P2A-dCas9-VPR

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pJet1.2 ermB cassette

Plasmid

#117121

Purpose

Erythromycin resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Erythromycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109

Plasmid

#111730

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q109 (polyQ repeat)

Promoter

CMV and P10

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-140

Plasmid

#118556

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-GFP-HYGRO

Plasmid

#115144

Purpose

Safe harbor site 231 knock-in vector with GFP-HygromycinR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3B-BE3

Plasmid

#113411

Purpose

Expresses hA3B-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3B-BE3 (APOBEC3B Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-FAM134B-LIRmut

Plasmid

#109027

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

FAM134B-LIRmut (RETREG1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

DDFELL to DDAEAL aa 453-458 to interrupt FAM134B …

Promoter

EIF-1a promoter

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23

Plasmid

#111723

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q23 (polyQ repeat)

Promoter

CMV and P10

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK CA RhoA

Plasmid

#112894

Purpose

promoter-inducible expression of constitutively-active RhoA

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

CA RhoA (RHOA Human)

Use

Lentiviral

Expression

Bacterial

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSET-dCas9

Plasmid

#110183

Purpose

Expression of the dCas9 gene in Streptomyces

Depositor

Yinhua Lu

Article

Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

Insert

dCas9

Use

CRISPR; I

Expression

Bacterial

Mutation

D10A, H840A

Promoter

ermE*p

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Htt171-66Q-myc-WPRE

Plasmid

#107934

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons

Depositor

Johan Jakobsson

Article

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

Exon 1 of mutant HTT, 66 polyQ repeats (HTT Human)

Use

AAV

Promoter

hSynapsin

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.A184S

Plasmid

#106186

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

CAG

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1RA-P2A-GFP-PGK-Puro

Plasmid

#110866

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pJJB308

Plasmid

#107699

Purpose

AtU6 Promoter-LwaCas13a-gRNA Scaffold

Depositor

Daniel Voytas

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Type

Empty backbone

Expression

Plant

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-tdTomatof

Plasmid

#104060

Purpose

Also known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracing

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

tdTomato

Use

AAV

Promoter

hSyn

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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