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We narrowed to 62,921 results for: cloning

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Showing: 8341 - 8360 of 62921 results

  1. pHmK-C1

    Plasmid
    #87866
    Purpose
    Backbone plasmid for expressing pHluorin-mKate2-fusion protein in mammalan cells, which contains multi-cloning site like pEGFP-C1
    Depositor
    Isei Tanida
    Publication
    Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi
    Insert
    pHluorin-mKate2 fusion
    Tags
    pHluorin-mKate2 fusion
    Expression
    Mammalian
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    87866-plasmid-map-sequence-id-252335

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  2. pRVL-3

    Plasmid
    #104581
    Purpose
    Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Publication
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and E318A/K320A/K322A
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    104581-plasmid-map-sequence-id-201392

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  3. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Publication
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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  16. pBP-lacZ

    Plasmid
    #72948
    Purpose
    Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning
    Depositor
    Paul Freemont
    Publication
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - Bioparts
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    72948-plasmid-map-sequence-id-133793

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Showing: 8341 - 8360 of 62921 results