We narrowed to 11,803 results for: nsf

1. pAAV-IGK-dAPEX2-KDEL

   Plasmid

   #117182

   Purpose

   Plasmid name in publication: pAAV-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   IGK-dAPEX2-KDEL
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-haA3A-CBE-G-C

   Plasmid

   #220899

   Purpose

   AAV genome encoding the C-terminal of haA3A-CBE-G, and sgRNA

   Depositor

   Dali Li

   Article

   Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

   Insert

   haA3A-CBE-G-C
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

   Plasmid

   #186993

   Purpose

   AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-s1006-gggs-d122, non-targeting crRNA guide
   

   Use

   AAV

   Mutation

   s1006-gggs-d1221 deletion

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB[nos-Cas9 PUb-ZsGreen]

   Plasmid

   #169011

   Purpose

   For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   Cas9
   

   Use

   Piggybac

   Expression

   Insect

   Promoter

   Drosophila melanogaster nanos

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-shRNA_Tet3

   Plasmid

   #85740

   Purpose

   shRNA against mouse Tet3

   Depositor

   Hongjun Song

   Article

   Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

   Insert

   Tet3 (Tet3 Mouse)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH

   Plasmid

   #74289

   Purpose

   pAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a Cre-dependent manner

   Depositor

   Edward Callaway

   Article

   Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

   Insert

   H2B-GFP and oG (optimized Glycoprotein)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   chimeric glycoprotein

   Promoter

   Ef1a

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1alpha-soCoChR-tdTomato

   Plasmid

   #107714

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Insert

   soCoChR-tdTomato
   

   Use

   AAV

   Tags

   KA2 and tdTomato

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-GRAB_eCBmut

   Plasmid

   #164605

   Purpose

   Express the endocannabinoid non-responsive sensor GRAB_eCBmut in neurons

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid non-responsive sensor GRAB_eCBmut
   

   Use

   AAV

   Mutation

   S383T, F177A

   Promoter

   hSyn

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.Syn.GCaMP6m.WPRE.SV40

   Plasmid

   #100841

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6m
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. RNF130 shRNA-TRE

    Plasmid

    #225336

    Purpose

    shRNAmir backbone under tet-operator for RNA interference, with YFP

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    RNF130 shRNA (Rnf130 Mouse)
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    TRE (tetracycline-responsive element)

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Per2:iLuc

    Plasmid

    #160293

    Purpose

    target Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.

    Depositor

    Joseph Takahashi

    Article

    Shan et al Neuron. 2020 Aug 4. pii: S0896-6273(20)3

    Insert

    Per2LUC
    

    Expression

    Mammalian

    Promoter

    no promotor

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-fDIO-VARNAM2-Kv2.1PR

    Plasmid

    #195536

    Purpose

    Flp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    CAG

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS100 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-TEV-MCS

    Plasmid

    #199353

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-TEV-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA-TEV

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMKII-SomArchon

    Plasmid

    #126942

    Purpose

    Expression of SomArchon under CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine)

    Plasmid

    #219426

    Purpose

    Cre-dependent expression of luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

    Insert

    mNeonGreen, ISN-IE, ChRmine
    

    Use

    AAV

    Tags

    Gaussia luciferase signal sequence

    Promoter

    EF-1alpha

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-pMecp2-SaCas9-pU6-sgRNA

    Plasmid

    #159173

    Purpose

    Vector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EFS-fDIO-GRAB_eCB2.0

    Plasmid

    #164610

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cells

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    EFS

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEINO1

    Plasmid

    #179472

    Purpose

    Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation

    Depositor

    Bogumil Karas

    Article

    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

    Inserts

    ori
    cat
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKII-VARNAM2-Kv2.1PR

    Plasmid

    #195530

    Purpose

    Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    CaMKII

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PZac2.1 hSyn1-HA-BioID2-SAPAP3

    Plasmid

    #176857

    Purpose

    Expresses SAPAP3 protein fused with BioID2 at the N-terminus in neurons

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    HA-BioID2-SAPAP3
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Promoter

    hSyn1

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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