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Plasmid#140027PurposeTunable and temporal expression control of nuclear eGFPDepositorInsertEnhanced Green Fluorescent Protein
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVtight eGFP Neo (w784-1)
Plasmid#26586Purpose2nd generation lentiviral vectorDepositorInsertEnhanced Green Fluorescent Protein
UseLentiviral; Tet-on advanced vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
HRE:GFP-pGK:Cre
Plasmid#68552PurposeLentivirus expressing hypoxia-responsive GFP reporter and CreDepositorInsertsGreen Fluorescent Protein
Cre
UseLentiviralTagsExpressionMammalianMutationPromoterHRE and pGKAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-AID-EGFP
Plasmid#80076PurposeMammalian expression vector for C-terminal AID-EGFP taggingDepositorInsertAID-EGFP
UseTagsAID and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-AID
Plasmid#80075PurposeMammalian expression vector for N-terminal EGFP-AID taggingDepositorInsertEGFP-AID
UseTagsAID and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSG25-pBAD-tetO-deGFPssrA
Plasmid#102452PurposeExpresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.DepositorInsertdeGFP
UseTagsssrAExpressionBacterialMutationPromoterpBAD-tetOAvailable sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG81-pLas-tetO-deGFPssrA
Plasmid#102454PurposeExpresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.DepositorInsertdeGFP
UseTagsssrAExpressionBacterialMutationPromoterpLas-tetOAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/er-(n2)oxStayGold(c4)v2.0
Plasmid#186296PurposeA new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.DepositorInserter-(n2)oxStayGold(c4)v2.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRuby2-MAPTau-C-10
Plasmid#55904PurposeLocalization: Microtubules, Excitation: 559, Emission: 600DepositorInsertMAPTau (MAPT Human)
UseTagsmRuby2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Gfp-zf-vasa
Plasmid#167323PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.DepositorInsertzf vasa 3'UTRs (ddx4 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
mRuby2-LaminA-C-18
Plasmid#55901PurposeLocalization: Nuclear Envelope, Excitation: 559, Emission: 600DepositorInsertLaminA (LMNA Human)
UseTagsmRuby2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mRuby2-Farnesyl-5
Plasmid#55896PurposeLocalization: Membrane, Excitation: 559, Emission: 600DepositorInsertFarnesyl (HRAS Human)
UseTagsmRuby2ExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pREF-GFP kan
Plasmid#122061Purposereporter plasmid for termination and terminator override to express GFP. Marked with URA3DepositorInsertsintergenic terminator from yeast IMD2
green fluorescent protein
UseTagsExpressionYeastMutationPromoterGAL1 and GAL1 comes in pYES2Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS2
Plasmid#12389DepositorInsertgreen fluorescent protein
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAS1
Plasmid#12388DepositorInsertgreen fluorescent protein
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Y203)
Plasmid#63559PurposeExpression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Y203)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged threonine at position 203 with respect to…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GB-NES
Plasmid#61017PurposeddGFP B-NESDepositorInsertddGFP B
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cx30-msfGFP
Plasmid#69019PurposeExpresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorInsertCx30 (GJB6 Human)
UseTagsV206K monomerized super folder GFPExpressionMammalianMutationPromotercAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(H66)
Plasmid#63558PurposeExpression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(H66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(W66)
Plasmid#63217PurposeExpression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(W66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only