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pBEL2533

Plasmid

#195654

Purpose

Complementation of MlaC(N179E)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

MlaC(N179E)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2534

Plasmid

#195655

Purpose

Complementation of MlaC(Q115A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

MlaC(Q115A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2535

Plasmid

#195656

Purpose

Complementation of MlaC(R134E)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

MlaC(R134E)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2536

Plasmid

#195657

Purpose

Complementation of MlaC(R147D)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

MlaC(R147D)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2640

Plasmid

#195629

Purpose

Complementation of MlaA(L245N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Mutation

MlaA(L245N)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2641

Plasmid

#195630

Purpose

Complementation of MlaA(I248N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Mutation

MlaA(I248N)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2644

Plasmid

#195631

Purpose

Complementation of MlaA(D198N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Mutation

MlaA(D198N)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2663

Plasmid

#195632

Purpose

Complementation of MlaA(F223N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Mutation

MlaA(F223N)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2664

Plasmid

#195633

Purpose

Complementation of MlaA(L230N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Mutation

MlaA(L230N)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2665

Plasmid

#195634

Purpose

Complementation of MlaA(delta244-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Mutation

MlaA(delta244-251)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2521

Plasmid

#195643

Purpose

Complementation of MlaC(A34R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

MlaC(A34R)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2512

Plasmid

#195626

Purpose

Complementation of MlaA(delta238-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Mutation

MlaA(delta238-251)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2513

Plasmid

#195627

Purpose

Complementation of MlaA(delta227-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Mutation

MlaA(delta227-251)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2639

Plasmid

#195628

Purpose

Complementation of MlaA(I241N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Mutation

MlaA(I241N)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1160

Plasmid

#196025

Purpose

Expression/purification of 6xHis-TEV-MlaD

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1161

Plasmid

#196026

Purpose

Expression/purification of 6xHis-TEV-MlaDdelta141-183

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaDdelta141-183

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1195

Plasmid

#196027

Purpose

Complementation of MlaF-MlaE-MlaD-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-NcPAN2_1-461_Y

Plasmid

#148104

Purpose

Bacterial Expression of NcPAN2_1-461

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

NcPAN2_1-461

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GB2566

Plasmid

#193104

Purpose

Minimal promoter of SlDFR gene containing 62bp upstream the transcription start site and the 5'UTR region.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minSlDFR

Use

Synthetic Biology

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPpT4- HTA1prom-TIR1-FLAG

Plasmid

#189725

Purpose

Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

HTA1

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPpT4- TEF2prom-TIR1-FLAG

Plasmid

#189726

Purpose

Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

TEF2

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
InterTag-1xL

Plasmid

#186911

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance gene

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-(GGGGS)1-PDGFR

Expression

Mammalian

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTopo RE2 Donor pLox hPGK-Puro pA pLox

Plasmid

#171049

Purpose

Donor template for CRISPR/Cas9 targeting of TERT RE2

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Puromycin

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-L3-Csy4-Cas9-EGFP-L2

Plasmid

#141045

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Csy4-2A-Cas9-2A-EGFP

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-L3-Csy4-FokI-dCas9-L2

Plasmid

#141042

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Csy4-2A-FokI-dCas9

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-L3-Cas9n-EGFP-L2

Plasmid

#141044

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9n-2A-EGFP

Expression

Mammalian

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-L3-Csy4-Cas9n-L2

Plasmid

#141041

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Csy4-2A-Cas9n

Expression

Mammalian

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-L3-Cas9-EGFP-L2

Plasmid

#141043

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9-2A-EGFP

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-L3-SpCas9-HF4-2A-EGFP-L2

Plasmid

#141049

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

SpCas9-HF4-2A-EGFP

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-L3-Csy4-Cas9-L2

Plasmid

#141040

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Csy4-2A-Cas9

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2

Plasmid

#91802

Purpose

Expresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be used

Depositor

Jeremy Wilusz

Article

Liang et al Methods. 2021 Apr 18. pii: S1046-2023(21

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-lyzC-microRNA 19c-Dendra2

Plasmid

#97104

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 19c Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pIVT/Acrosin-GFP-GPI

Plasmid

#122141

Purpose

For making acrosin-GFP-GPI RNA

Depositor

Carmen Williams

Article

Winuthayanon et al Elife. 2015 Dec 1;4:e10453. doi: 10.7554

Insert

acrosin-GFP-GPI

Use

For in vitro transcription

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRG820-estrella

Plasmid

#99104

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea estrella

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

estrella in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM1886.3

Plasmid

#84104

Purpose

target miRNA1886.3 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM1886.3

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
tdminiSOG-MAP4-C-10

Plasmid

#57805

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

MAP4

Tags

tdminiSOG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pFOSGE_COF3G08

Plasmid

#71044

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Hrb98DE (Hrb98DE Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4A06

Plasmid

#71046

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Uba1 (Uba1 Fly)

Use

Gateway entry vector

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR H1 L1-L4

Plasmid

#62104

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p075 pCS xTRPS1 delN delC119 (M)

Plasmid

#11045

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 delN delC119 mut (trps1 Frog)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

aa806-1153 of xTRPS1. Two Cys residues in the GA…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
p076 pCS xTRPS1 delN delC320 (M)

Plasmid

#11046

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 (trps1 Frog)

Use

Mammalian, avian, and zebrafish

Mutation

aa806-952 of xTRPS1. Two Cys residues in the GAT…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-VASP-5

Plasmid

#55098

Purpose

Localization: Actin/Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

VASP

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pIDTsmart-MbPylRS-4xPyOtR-ITR-GFP

Plasmid

#240124

Purpose

Express AAV2 transgene (EGFP) and MbPylRS/tRNA for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Pham et al Mol Ther Oncol. 2025 Aug 23;33(4):201040

Inserts

AAV2-ITR-EGFP
MbPylRS
PylOtR

Use

AAV

Expression

Mammalian

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-LMP1

Plasmid

#195805

Purpose

Gateway Entry Clone of Epstein-Barr virus LMP1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

LMP1 (LMP-1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKB01_mCherry

Plasmid

#69104

Purpose

Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-EBNA1

Plasmid

#195827

Purpose

Gateway Entry Clone of Epstein-Barr virus EBNA1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNA1 (EBNA-1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
7Gli:GFP

Plasmid

#110494

Purpose

reporter of Hedgehog signaling activity

Depositor

Michael Lewis

Article

Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

Insert

7 repeats of the Gli binding site GAACACCCA

Use

Lentiviral

Expression

Mammalian

Promoter

7 repeats of Gli biding site

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-BALF2

Plasmid

#198578

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BALF2 (BALF2 Epstein-Barr virus)

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-BARF1

Plasmid

#195783

Purpose

Gateway Entry Clone of Epstein-Barr virus BARF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BARF1 (BaRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-LMP2A

Plasmid

#195816

Purpose

Gateway Entry Clone of Epstein-Barr virus LMP2A

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

LMP2A (LMP-2A Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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