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Showing: 2051 - 2100 of 13356 results
  1. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid
    #229767
    Purpose
    Human Col4A3 tagged at N-terminal with SmBiT to be used with N-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system
    Depositor
    Insert
    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    Use
    Luciferase
    Tags
    SmBiT
    Mutation
    See Depositor Comments
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid
    #61362
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pαH-S-GSAS-OMICRON.BA.2-RBD

    Plasmid
    #184830
    Purpose
    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Insert
    S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    6X His tag
    Expression
    Mammalian
    Mutation
    SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pαH-S-GSAS-OMICRON.BA.1-RBD

    Plasmid
    #184831
    Purpose
    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Insert
    S-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    6X His tag
    Expression
    Mammalian
    Mutation
    SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  9. pαH-rS2d

    Plasmid
    #183516
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein S-GSAS-rS2d with (682-685) furin side replaced with GSAS and mutation S383C, D985C
    Depositor
    Insert
    rS2d (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    May 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid
    #61361
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  12. pαH-rS2d-HexaPro

    Plasmid
    #183515
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P
    Depositor
    Insert
    Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    May 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pαH-S-GSAS/XBB.1.5

    Plasmid
    #212991
    Purpose
    "Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 variant
    Depositor
    Insert
    pαH-S-GSAS/XBB.1.5 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    (T19I,Del24/26,A27S,V83A,G142D,Del144,H146Q,Q183E…
    Promoter
    CMV
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. pαH-S-RRAR-OMICRON

    Plasmid
    #180424
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side intact
    Depositor
    Insert
    Spike (S-RRAR-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pαH-S-GSAS-OMICRON.BA.2

    Plasmid
    #184829
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Insert
    S-GSAS-OMICRON.BA.2 (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pαH-S-GSAS-B.1.617.2.v1

    Plasmid
    #182575
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein Delta variant, furin side replaced by GSAS, 2 Proline at 986 and 987 plus T19R-G142D-del156/157-R158G-L452R-T478K-D614G-P681R-D950N
    Depositor
    Insert
    Spike S-GSAS-B.1.617.2.v1 (T19R-G142D-Del156/157-R158G-L452R-T478K-D614G-P681R-D950N) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. pαH-S-RRAR/XBB.1.16

    Plasmid
    #213037
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.16 with with furin side intact
    Depositor
    Insert
    s -RRAR.XBB.1.16 (S )
    Tags
    HRV 3C cleavage site (before tags) 8X His tag 2X…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 furin site RRAR …
    Promoter
    CMV
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. pαH-S-RRAR-OMICRON.BA.5

    Plasmid
    #213071
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 with with furin side intact
    Depositor
    Insert
    pαH-S-RRAR-OMICRON.BA.5 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685, furin site inta…
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  19. pαH-S-GSAS/EG.5.1

    Plasmid
    #218668
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG.5.1 variant
    Depositor
    Insert
    pαH-S-GSAS/EG.5.1 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    CMV
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  20. pαH-S-GSAS/EG.5

    Plasmid
    #212990
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG5 variant
    Depositor
    Insert
    pαH-S-GSAS/EG.5 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    CMV
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. pαH-S-GSAS-2P-OMICRON

    Plasmid
    #180593
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Insert
    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  22. pαH-S-GSAS-OMICRON

    Plasmid
    #180423
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS
    Depositor
    Insert
    Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
  23. pαH-S-RRAR/XBB.1.5

    Plasmid
    #212993
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 with with furin side intact
    Depositor
    Insert
    Spike S-RRAR/XBB.1.5 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); furin site intake; (T19I…
    Promoter
    CMV
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  24. FLX1.8MitoKaede

    Plasmid
    #28133
    Depositor
    Insert
    MitoKaede
    Use
    Lentiviral
    Tags
    COX VIII signal sequence
    Expression
    Mammalian
    Available Since
    Oct. 14, 2011
    Availability
    Academic Institutions and Nonprofits only
  25. pPI54

    Plasmid
    #113197
    Purpose
    extrachromosomal expression vector, N-terminal mTurquoise2 tagging
    Depositor
    Type
    Empty backbone
    Use
    Dictyostelium discoideum expression vector, extra…
    Tags
    mTurquoise2
    Available Since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  26. pPI142

    Plasmid
    #113198
    Purpose
    extrachromosomal expression vector, C-terminal mTurquoise2 tagging
    Depositor
    Type
    Empty backbone
    Use
    Dictyostelium discoideum expression vector, extra…
    Tags
    mTurquoise2
    Available Since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  27. XP12 Xbra

    Plasmid
    #16978
    Depositor
    Insert
    Xbra (t.S Frog)
    Expression
    Bacterial
    Available Since
    Feb. 1, 2008
    Availability
    Academic Institutions and Nonprofits only
  28. pLenti6.2_miRFP670

    Plasmid
    #113726
    Purpose
    Expresses the fluorescent protein miRFP670 in a lentiviral backbone
    Depositor
    Insert
    miRFP670
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  29. pCSIIbleo-iRFP670-P2A-EGFP

    Plasmid
    #198060
    Purpose
    The lentiviral vector for iRFP670 and EGFP
    Depositor
    Insert
    iRFP670
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  30. pCSIIbleo-miRFP670nano-P2A-EGFP

    Plasmid
    #198064
    Purpose
    The lentiviral vector for miRFP670nano and EGFP
    Depositor
    Insert
    miRFP670nano
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  31. pGLO-Blue

    Plasmid
    #220782
    Purpose
    Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)
    Depositor
    Insert
    blue chromoprotein (aeBlue)
    Tags
    8xHis
    Expression
    Bacterial
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
  32. pLenti6.2_TagBFP

    Plasmid
    #113724
    Purpose
    Expresses the fluorescent protein TagBFP in a lentiviral backbone
    Depositor
    Insert
    TagBFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  33. pLenti6.2_mTurquoise2

    Plasmid
    #113723
    Purpose
    Expresses the fluorescent protein mTurquoise2 in a lentiviral backbone
    Depositor
    Insert
    mTurquoise2
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  34. CTV

    Plasmid
    #131590
    Purpose
    Plasmid for the targeting of chloramphenicol gene
    Depositor
    Insert
    Neomycin resistance gene
    Expression
    Bacterial
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  35. Protein2PAM_NmeQ981A

    Plasmid
    #250230
    Purpose
    Express Protein2PAM_NmeQ981A nuclease in human cells using a CMV promoter
    Depositor
    Insert
    NmeQ981A
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 Q981A
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
  36. Protein2PAM_NmeN1029A

    Plasmid
    #250229
    Purpose
    Express Protein2PAM_NmeN1029A nuclease in human cells using a CMV promoter
    Depositor
    Insert
    NmeN1029A
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 N1029A
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
  37. pY119 (pcDNA3.1-huLb5Cpf1)

    Plasmid
    #92295
    Purpose
    Expression of humanized Lb5Cpf1
    Depositor
    Insert
    huLb5Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
  38. pY118 (pcDNA3.1-huLb4Cpf1)

    Plasmid
    #92294
    Purpose
    Expression of humanized Lb4Cpf1
    Depositor
    Insert
    huLb4Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
  39. pCSIIbleo-NIR-GECO2-P2A-EGFP

    Plasmid
    #198063
    Purpose
    The lentiviral vector for NIR-GECO2 and EGFP
    Depositor
    Insert
    NIR-GECO2
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  40. pCSIIbleo-NIR-GECO1-P2A-EGFP

    Plasmid
    #198065
    Purpose
    The lentiviral vector for NIR-GECO1 and EGFP
    Depositor
    Insert
    NIR-GECO1
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  41. pGLO-Pink-SHE

    Plasmid
    #220781
    Purpose
    Expresses a raspberry chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)
    Depositor
    Insert
    raspberry chromoprotein (mRFP1)
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 2051 - 2100 of 13356 results