We narrowed to 12,362 results for: NUC

1. pICE-HA-MRE11-H129N

   Plasmid

   #82034

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of H129N nuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Nuclease-dead mutant: H129N MRE11

   Promoter

   CMV-tet

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICE-HA-MRE11-H63D

   Plasmid

   #82036

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Exonuclease-dead mutant: H63D MRE11

   Promoter

   CMV-tet

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-HP1alpha

   Plasmid

   #179703

   Purpose

   visualization of centromere heterochromatin

   Depositor

   Kenji Sugimoto

   Article

   Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

   Insert

   heterochromatin protein alpha (CBX5 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Drosha

   Plasmid

   #62520

   Purpose

   wild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sites

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha full length (DROSHA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6

   Plasmid

   #177849

   Purpose

   Bacterial Expression of PNR

   Depositor

   Karsten Melcher

   Article

   Tan et al PLoS One. 2013 Sep 12;8(9):e74359. doi:

   Insert

   photoreceptor cell-specific nuclear receptor (NR2E3 Human)
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMH-SFB-DYRK1A

   Plasmid

   #101770

   Purpose

   Mammalian expression of DYRK1A fusion protein

   Depositor

   Michael Huen

   Article

   Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

   Insert

   DYRK1A (DYRK1A Human)
   

   Use

   Tags

   S peptide-flag-streptavidin binding peptide (SFB)

   Expression

   Mammalian

   Mutation

   siRNA-resistant allele

   Promoter

   Available since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-DsRed_IRES_MAPT:EGFP

   Plasmid

   #92196

   Purpose

   Lentiviral overexpression vector to make stable bicistronic cell line for Tau screen

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   DsRed-IRES-MAPT-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-7 aSyn C141

   Plasmid

   #108866

   Purpose

   Expresses human WT alpha synuclein with a C-terminal cysteine

   Depositor

   Gabriele Kaminski Schierle

   Article

   Stephens et al bioRxiv 2022.10.04.510646

   Insert

   alpha synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C141 mutation to allow C-terminal maleimide dye l…

   Promoter

   T7

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-GFP-IRES-hSNCA

   Plasmid

   #185715

   Purpose

   AAV expression of GFP and human α-Synuclein from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro

    Plasmid

    #185451

    Purpose

    Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

    Insert

    Nucleocapsid (N SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MLF2-GFP-3xFLAG

    Plasmid

    #230986

    Purpose

    Expresses GFP tagged MLF2 in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

    Insert

    MLF2 (MLF2 Human)
    

    Use

    Tags

    3xFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCVL.SSA TLR (Sce)

    Plasmid

    #45576

    Purpose

    Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    5' iRFP arm
    eGFP with I-Sce I TS
    +3 mCherry
    3' iRFP arm
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185bp, truncated 25 …

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-sACt-mCherry-NLS

    Plasmid

    #138214

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NLS (Adcy10 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PEA1

    Plasmid

    #176527

    Purpose

    Delivers all prime editing nickase components in a single plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CbH for Cas9, hU6 for gRNAs

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-GFP-IRES-mSNCA

    Plasmid

    #185714

    Purpose

    AAV expression of GFP and mouse α-Synuclein from hSyn promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    synuclein, alpha (Snca Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-UAP56

    Plasmid

    #157661

    Purpose

    Expresses UAP56 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Spliceosome RNA helicase DDX39B (DDX39B Human)
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-43

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-EF1a-V5-Lem2

    Plasmid

    #120245

    Purpose

    Expresses V5-tagged Lem2 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Lem2 (Lemd2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV6-G1-ATP8-Myc-FLAG (G418)

    Plasmid

    #86850

    Purpose

    mammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tags

    Depositor

    Matthew O'Connor

    Article

    Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

    Insert

    ATP8 (ATP8 Human)
    

    Use

    Tags

    ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. shFGF2#2

    Plasmid

    #157668

    Purpose

    Tet inducible knockdown of FGF2

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-E3CY56

    Plasmid

    #103075

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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