We narrowed to 2,200 results for: dcas9

1. p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR

   Plasmid

   #186745

   Purpose

   dCas9-10XGcn4-P2A-mCherry expression construct

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   Cas9-10XGcn4-P2A-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS

   Plasmid

   #107323

   Purpose

   Expresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-NmCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 (R1335K) is fused to nuclease dead NmCas9 …

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. KN801: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-VPR

   Plasmid

   #121829

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/VPR (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRAB

   Plasmid

   #121830

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/KRAB (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. KO001: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-p300core

   Plasmid

   #121832

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/p300core (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101115

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to LPAR1

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   LPAR1-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101113

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to CXCR4

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   CXCR4-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

   Plasmid

   #101114

   Purpose

   Encodes dCas9(N) fused to CXCR4

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   CXCR4-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

   Plasmid

   #172317

   Purpose

   CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101111

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to AVPR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    AVPR2-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAVPR2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101112

    Purpose

    Encodes dCas9(N) fused to AVPR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    AVPR2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE

    Plasmid

    #101106

    Purpose

    Encodes FKBP-dCas9(N) fused to VEGFR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    VEGFR2-FKBP-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64

    Plasmid

    #101110

    Purpose

    Encodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(C)_DHFR-PCP-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101116

    Purpose

    Encodes dCas9(N) fused to LPAR1

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    LPAR1-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. nAPEX2-V5-zdCas9n-3x-FLAG-GSG-linker-P2A-mNeongreen

    Plasmid

    #241191

    Purpose

    Middle entry vector for mini-Golden subcloning platform; 3x-FLAG; mNeongreen

    Depositor

    Junsu Kang

    Insert

    3x-FLAG; mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 3x-FLAG-nzdCas9-V5-APEX2n-GSG-linker-P2A-mNeongreen

    Plasmid

    #241190

    Purpose

    Middle entry vector for mini-Golden subcloning platform; V5-APEX2n; mNeongreen

    Depositor

    Junsu Kang

    Insert

    V5-APEX2n; mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP

    Plasmid

    #227009

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mutation

    Promoter

    UbC

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP

    Plasmid

    #227010

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mutation

    Promoter

    UbC

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP

    Plasmid

    #227011

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mutation

    Promoter

    UbC

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

    Plasmid

    #227012

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mutation

    Promoter

    UbC

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge