Addgene

We narrowed to 14,735 results for: invs

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pRS406-PHO5-GFP-hFAPP1 PH domain

Plasmid

#58723

Purpose

Expresses GFP-Tagged human FAPP1 PH domain in yeast

Depositor

Tim Levine

Article

Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

Insert

Four-phosphate-adaptor protein 1 PH domain (PLEKHA3 Human)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pSC6

Plasmid

#104780

Purpose

pSC218UG-At Ubiquitin10:Gateway expression cassette binary vector

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLinker-AID-3xHA-DHFR-LoxP

Plasmid

#86669

Purpose

for generation of AID strains using CRISPR tagging technology

Depositor

David Sibley

Article

Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

Insert

Linker-AID-3xHA-HXGPRT 3'UTR

Use

Expression in toxoplasma gondii

Tags

Expression

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-mTagBFP-CAAX

Plasmid

#75176

Purpose

Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mTagBFP-CAAX

Use

Three prime entry vector for multisite gateway th…

Tags

Expression

Mutation

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLinker-AID-Ty-HXGPRT-LoxP

Plasmid

#86667

Purpose

for generation of AID strains using CRISPR tagging technology

Depositor

David Sibley

Article

Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

Insert

Linker-AID-Ty-HGPRT 3'UTR

Use

Expression in toxoplasma gondii

Tags

Expression

Mutation

Promoter

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP_Rab8a

Plasmid

#31803

Depositor

Daniel Gerlich

Article

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

Rab8a (RAB8A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pSC5

Plasmid

#104779

Purpose

pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mCerulean3

Plasmid

#75156

Purpose

Nuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pWJB30

Plasmid

#126505

Purpose

Salmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30

Depositor

Ute Romling

Article

Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

Insert

adrA-8xHis

Use

Tags

8xHis

Expression

Bacterial

Mutation

Promoter

PBAD30

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LbCpf1-U6_FlipArray

Plasmid

#109349

Purpose

Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

Depositor

Sidi Chen

Article

Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

Insert

U6-FlipArray

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Blast NT shRNA

Plasmid

#123306

Purpose

Expression of a non-targeting shRNA

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

Non-targeting shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mt-ro2GFP

Plasmid

#82408

Purpose

Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro2GFP

Use

Tags

leader sequence of the E1α subunit of pyruvate de…

Expression

Mammalian

Mutation

C48S/F64L/S147C/Q204C

Promoter

CMV

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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NPC1-CFP P692S

Plasmid

#58457

Purpose

expression of NPC1-CFP sterol sensing domain mutant

Depositor

Matthew Scott

Article

Ko et al Mol Biol Cell. 2001 Mar;12(3):601-14.

Insert

NPC1 (Npc1 Mouse)

Use

Tags

ECFP and His6

Expression

Mammalian

Mutation

P692S sterol sensing domain mutant

Promoter

CMV

Available since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
MAGE-A3Pro pGL4basic

Plasmid

#78138

Purpose

luciferase reporter plasmid

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MAGE-A3 (MAGEA3 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains -418/+12 of promoter

Promoter

MAGE-A3

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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PL452-hGH-polyA-PGK-neoR

Plasmid

#191681

Purpose

Donor plasmid backbone PGK-neoR

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAR3040-spitz

Plasmid

#38341

Depositor

Norbert Perrimon

Article

Rutledge et al Genes Dev. 1992 Aug;6(8):1503-17.

Insert

spitz (spi Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pCS2-Opto-Alk8

Plasmid

#207615

Purpose

Zebrafish Alk8 BMP receptor kinase domain fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

Alk8 (acvr1l) kinase domain + VfLOV domain (acvr1l Zebrafish, Vaucheria frigida)

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-NS-MCS

Plasmid

#75179

Purpose

mCherry (red) fluorophore with multiple cloning site for use with inducible N-terminal fusions.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mCherry-NS-MCS

Use

Three prime entry vector for multisite gateway th…

Tags

Expression

Mutation

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPHAGE2-Myc-mTZAP

Plasmid

#87188

Purpose

lentiviral stable expression of Myc-TZAP (mouse) in mouse embryonic stem cells

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (Zbtb48 Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

unknown

Available since

March 10, 2017

Availability

Academic Institutions and Nonprofits only

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pME-mKOFP2-CAAX

Plasmid

#75155

Purpose

Membrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mKOFP2-CAAX

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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