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pYES2/103Q

Plasmid

#1385

Depositor

Michael Sherman

Article

Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

Insert

huntingtin (103Q) (HTT Human)

Tags

EGFP and FLAG

Expression

Yeast

Mutation

Exon1 sequences containing the first 17 amino aci…

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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ZapLck biosensor

Plasmid

#131584

Purpose

Lck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Wan et al Sci Adv. 2019 Jun 19;5(6):eaau2001. doi:

Insert

ZapLck biosensor

Use

Lentiviral

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Nurr1

Plasmid

#35000

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Nuclear receptor subfamily 4 group A member 2 (Nr4a2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pYES2/25Q

Plasmid

#1384

Depositor

Michael Sherman

Article

Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

Insert

huntingtin (25Q) (HTT Human)

Tags

EGFP and FLAG

Expression

Yeast

Mutation

Exon1 sequences containing the first 17 amino aci…

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT5-d2EGFP-mCherry

Plasmid

#201454

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT5_d2EGFP

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBA407-Neo-COVGT5-mCherry

Plasmid

#201455

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT5_mCherry

Use

Lentiviral and Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLR5-CBh-dCas9-hEzh2-IRES-Hyg

Plasmid

#122375

Purpose

Expresses dCas9-hEzh2-IRES-Hyg in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

CBh-dCas9-hEzh2-IRES-Hyg-pA

Use

Piggybac

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBP-split-intein-Gal4[1-20]-N-int

Plasmid

#199201

Purpose

Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[1-20]-N-int

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCAX-EGFP-FBP17

Plasmid

#178365

Purpose

Expresses FBP17 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Formin Binding Protein 17 (FNBP1 Human)

Expression

Mammalian

Promoter

Chicken Beta Actin

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pXJ40 GADD34 (514-674aa)

Plasmid

#75480

Purpose

mammalian expression of GADD34 514-674 aa

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 514-674

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
pXJ40 GADD34 (1-513aa)

Plasmid

#75479

Purpose

mammalian expression of GADD34 1-513 aa

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 1-513

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
px335-sgRNA-EF(Cas9n)-Dnmt3b-R

Plasmid

#122333

Purpose

Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Dnmt3b

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRalpha

Plasmid

#136434

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRA

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRalpha (PDGFRA Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Dnmt3b-L

Plasmid

#122334

Purpose

Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Dnmt3b

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1-V5-HisB-ObKHC-wt-HaloTag

Plasmid

#201551

Purpose

Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Kinesin-1 (partial)

Tags

HaloTag

Expression

Insect

Mutation

Codon-optimized for Drosophila expression

Promoter

Ac5

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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plexm-human LRRC33

Plasmid

#111600

Purpose

Express human LRRC33 in 393T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

LRRC33 (NRROS Human)

Tags

FLAG

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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SNRPA_Full-length_pGEX

Plasmid

#135134

Purpose

Recombinant expression of SNRPA with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SNRPA (SNRPA Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Feb. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA pcDNA3

Plasmid

#206051

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 BBS pcDNA3

Plasmid

#206057

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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phage - ubc - nls - 2xmcp - egfp - BirA*

Plasmid

#131132

Purpose

BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

Depositor

Ralf Jansen

Article

Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11

Insert

NLS-HA-2xMCP-EGFP-BirA*

Use

Lentiviral

Tags

NLS, HA

Expression

Mammalian

Mutation

BirA* = BIrA >R118G

Promoter

human ubiquitin C (UBC)

Available Since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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