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Hmga2 3'UTR m2 luciferase (Luc-m2)

Plasmid

#14786

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Luc-m2 (Hmga2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

m2 mutant (see article and author's map for …

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pBabe HcRed Ras

Plasmid

#10678

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Insert

Hras V12 (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

G12V, constitutively active

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pCDF-NtXR1AtR2B2NtS1

Plasmid

#160879

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Use

Tags

Expression

Bacterial

Mutation

Promoter

rbcS-S1: pBAD, chaperones: T7

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMA-Thermal_CTLA-4

Plasmid

#199751

Purpose

Thermal expression of CTLA-4

Depositor

Mikhail Shapiro

Article

Abedi et al Nat Commun. 2022 Mar 24;13(1):1585. doi:

Insert

CTLA-4 (Ctla4 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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-2.5Kb MMP10

Plasmid

#53973

Purpose

Luciferase reporter for mouse MMP10 2.5kb promoter

Depositor

Michael Chin

Article

Wu et al Biochem Biophys Res Commun. 2011 Dec 2;4

Insert

mouse MMP10 2.5Kb promoter (Mmp10 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PCR from C57BL6/J

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS1R1AtR2B2

Plasmid

#160880

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Use

Tags

Expression

Bacterial

Mutation

Promoter

rbcS-S1: T7, chaperones: T7

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtTcR1AtR2NtB2

Plasmid

#160889

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-Tc

Use

Tags

Expression

Bacterial

Mutation

Promoter

rbcS-Tc: T7, chaperones: T12

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-hPDI

Plasmid

#60555

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Use

Tags

His6-hPDI

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtT2R1AtR2NtB2

Plasmid

#160886

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-T2

Use

Tags

Expression

Bacterial

Mutation

Promoter

rbcS-T2: T7, chaperones: T9

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-hPDIb′a′

Plasmid

#60556

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Use

Tags

His6-hPDIb'a' tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pIVT-EGFP-mouseCENP-C

Plasmid

#174444

Purpose

in vitro transcription template for mouse CENP-C (N-ter EGFP tagged)

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Centromere protein C

Use

In vitro transcription template

Tags

EGFP

Expression

Mutation

Promoter

T7 SP6

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV mTurquoise2-superYFP tandem

Plasmid

#157776

Purpose

Produces a fusion between mTurquoise2 and superYFP. It can be used as a positive control for FRET (using FLIM)

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Type

Empty backbone

Use

Tags

mTurquoise2 and superYFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Zfp36-3'UTR

Plasmid

#61780

Purpose

Luciferase reporter containing the 3'UTR of mouse Zfp36

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Zfp36 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pExodus

Plasmid

#39991

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV, T7

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

Plasmid

#45578

Purpose

Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

coY2 Ani

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) neo with Smad5 CDS

Plasmid

#61800

Purpose

pcDNA3.1(+) with full length mouse Smad5 CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Smad5 CDS (Smad5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
GST-SmB'

Plasmid

#1842

Depositor

Philip Leder

Article

Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

Insert

SmB' (SNRPB Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pGFPmAID_htz-1

Plasmid

#174214

Purpose

Donor plasmid for tagging htz-1 with GFP::mAID

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

GFP::mAID::htz-1 tagging donor

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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GST-SmB

Plasmid

#1841

Depositor

Philip Leder

Article

Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

Insert

SmB (SNRPB Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-DKAR-Nuc

Plasmid

#45499

Depositor

Alexandra Newton

Article

Bossuyt et al J Biol Chem. 2011 Sep 23;286(38):33390-4

Insert

DKAR-Nuc

Use

Tags

CFP, NLS, and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only