We narrowed to 927 results for: pgem
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C077
Plasmid#174346PurposeTemplate for making mRNA for overexpressing frog PABPC1 (C-FLAG) in frog oocytesDepositorAvailabilityAcademic Institutions and Nonprofits only -
pISH-Olfr156
Plasmid#139486PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH)DepositorInsertISH probe for Olfr156 - UTR (Olfr156 Mouse)
UseIsh probeAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISH-Vmn2r118
Plasmid#108086PurposePreparation of RNA probe for ISH of mouse Vmn2r118 geneDepositorInsertVmn2r118 (Vmn2r118 Mouse)
UseIn vitro transcriptionMutationchanged T to C at nt 830 of NM_001104582.1Available sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcFNE3A (chick fibronectin E3A)
Plasmid#14062DepositorInsertChicken Fibronectin EIIIA (FN1 Chicken)
ExpressionMammalianMutationBlunted BstE2-AvaII fragment was inserted into bl…Available sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
C233
Plasmid#174349PurposeTemplate for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytesDepositorAvailabilityAcademic Institutions and Nonprofits only -
pdCas9 (GB1079)
Plasmid#75399PurposeProvides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusionsDepositorInsertCas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-OMACS
Plasmid#136765PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Acsm4DepositorInsertAcsm4 (Acsm4 Mouse)
UseIn situ probeMutationISH Probe insert, A102T- please see depositor com…Available sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDS69
Plasmid#48367PurposeFLAG-intergenic region of TUB-HYG (modified from PMID 16269191)DepositorInsertFLAG-intergenic region of TUB-HYG (modified from PMID 16269191)
UseCre/Lox; Knockout in t. bruceiAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
C078
Plasmid#174347PurposeTemplate for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytesDepositorInsertePAB
UseIn vitro transcription templateTagsFLAGMutation13 silent mutations: G243A, A273G, G339A, C345G, …AvailabilityAcademic Institutions and Nonprofits only -
pAGH50
Plasmid#107254PurposeVipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.DepositorInsertvipp1-gfp-fkbp12
UseSuicide vector for destined for double homologous…TagsGFP and FKBP12ExpressionBacterialPromoternativeAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMGF130
Plasmid#96971PurposeS. pombe lem2 knockout cassette with hygro resistance geneDepositorInsertlem2 (lem2 Fission Yeast)
ExpressionBacterialMutationwhole ORF deletion and replaced with hygro resist…PromoterN/AAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMGF172
Plasmid#96998Purposeish1 mCherry insertion cassette with ura4+ geneDepositorInsertish1 (ish1 Fission Yeast)
TagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + ura4(…PromoterN/AAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pG-HIV-LRT
Plasmid#104592PurposeStandard for qPCRDepositorInsertHIV-1 late reverse transcripts
UseCloning vectorAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMGF169
Plasmid#96996Purposeish1 mCherry insertion cassette with G418 resistance geneDepositorInsertish1 (ish1 Fission Yeast)
TagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + G418 …PromoterN/AAvailable sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHsp18.2 (GB0153)
Plasmid#68171PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 promoter
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJC5-4-insulator-BREreporter-insulator
Plasmid#171499Purposeinjections to make Bmp reporter mouse transgenic lineDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertInsulator BRE-MLP:H2B-Turqoise
ExpressionMammalianPromoterBRE-MLPAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [D1_n-1] (GB1208)
Plasmid#75409PurposetRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)DepositorInserttRNA-gRNA position [D1_n-1]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
psgRNA (GB0645)
Plasmid#68210PurposeProvides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target siteDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSgRNA
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAT4
Plasmid#112580PurposeUsed for expression of protein in E. coli as non-fusionsDepositorTypeEmpty backboneExpressionBacterialAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
