159,157 results

1. pSL0283 (pTnsABC)

   Plasmid

   #130633

   Purpose

   Expresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTnsA, VchTnsB, VchTnsC
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. plenti.CMV.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

   Plasmid

   #236407

   Purpose

   Expresses cytosolic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS578_DD-dSpyCas9-mCherry-APEX2

   Plasmid

   #108570

   Purpose

   Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Insert

   dSpyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   APEX2, Destabilization domains, Flag, and mCherry…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDV052

   Plasmid

   #191825

   Purpose

   Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR

   Depositor

   Jim Roberts

   Article

   Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX307-TMPRSS2-puro

   Plasmid

   #158457

   Purpose

   Constitutive lentiviral expression of human TMPRSS2.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p323-L10A-PATagRFP

   Plasmid

   #74172

   Purpose

   Fluorescent reporter for single ribosome tracking

   Depositor

   Robert Singer

   Article

   Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

   Insert

   ribosomal protein L10A (Rpl10a Rat)
   

   Use

   Lentiviral

   Tags

   PATagRFP

   Expression

   Mutation

   Promoter

   UbC

   Available since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pREDCas9

   Plasmid

   #71541

   Purpose

   Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

   Depositor

   Tao Chen

   Article

   Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

   Inserts

   Cas9
   lambda red genes
   plasmid curing system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22b_PstS_1

   Plasmid

   #78198

   Purpose

   Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A197C (please see depositors comment below)

   Promoter

   T7

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIMAY

   Plasmid

   #68939

   Purpose

   E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

   Depositor

   Tim Foster

   Article

   Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

   Type

   Empty backbone

   Use

   Staphylococcal allelic exchange vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-TIS11B

    Plasmid

    #121157

    Purpose

    Mammalian expression of GFP tagged TIS11B

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    ZFP36L1 (TIS11B) (ZFP36L1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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