We narrowed to 23,621 results for: promoter

1. pGFP_6

   Plasmid

   #238194

   Purpose

   Empty lentiviral vector for expressing protein sequences fused to GFP-6mer_oligomerization_domain, under a TRE3GV promoter

   Depositor

   Feng Zhang

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP-6mer and SV40

   Expression

   Mammalian

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS313-rad53-5004

   Plasmid

   #232902

   Purpose

   Plasmid carrying the rad53-5004 allele with its native promoter and terminator.

   Depositor

   Jolanda Van Leeuwen

   Insert

   RAD53 (RAD53 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   T600C, A680G, A1094T, C1615T, C2228A

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-phSyn-flex-SypHaloTag

   Plasmid

   #172280

   Purpose

   Cre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.

   Depositor

   Danny Winder

   Insert

   synaptophysin-HaloTag
   

   Use

   AAV and Cre/Lox

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   rat synapsin

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)

   Plasmid

   #219689

   Purpose

   Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2PsB (mtagBFP2, mCherry, NeonGreen)
   

   Expression

   Plant

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. MUT_S1-N

   Plasmid

   #218398

   Purpose

   3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Expression

   Mammalian

   Promoter

   tetracycline-responsive promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MUT_N-H1

   Plasmid

   #218397

   Purpose

   3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Expression

   Mammalian

   Promoter

   tetracycline-responsive promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCT03

   Plasmid

   #212150

   Purpose

   Plasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.

   Depositor

   Kang Zhou

   Insert

   alanine dehydrogenase
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   PT7

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEV-G2-Km ymTurquoise2

   Plasmid

   #193958

   Purpose

   TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymTurquoise2 in budding yeast

   Depositor

   Bas Teusink

   Insert

   ymTurquoise2
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. p416-GPD-HA-PPK(mutant)

   Plasmid

   #183944

   Purpose

   Expresses HA-tagged catalytic mutant of E. coli PPK from yeast GPD promoter

   Depositor

   Michael Downey

   Insert

   PPK(mutant) (ppk E. coli)
   

   Tags

   HA

   Expression

   Yeast

   Mutation

   mutations in His435, His454, and His592 to Alanine

   Promoter

   GPD

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL1271

    Plasmid

    #129282

    Purpose

    Expression plasmid with pLL1270 as backbone and alpha-Xylp(Ga0256695_ 1211)

    Depositor

    Lee Lynd

    Insert

    alpha-Xylp_1211 (Ga0256695_ 1211)
    

    Expression

    Bacterial

    Promoter

    Clostridium thermocellum DSM1313 Cellobiose Phosp…

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. brr2-R1107M pRS313

    Plasmid

    #111417

    Purpose

    brr2-R1107M (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    R1107M aa mutation (note, not an RP allele)

    Availability

    Academic Institutions and Nonprofits only

    

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12. ppyCAG_RNaseH1_WKKD

    Plasmid

    #111905

    Purpose

    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…

    Available Since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer10b-EGFP-KRAS-WT

    Plasmid

    #164935

    Purpose

    Expresses EGFP cDNA and KRAS wild type cDNA in mammalian cells

    Depositor

    Ji Luo

    Article

    Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

    Inserts

    Enhanced green fluorescent protein
    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    

    Use

    Lentiviral

    Promoter

    Ubc Promoter and Ubc promoter

    Available Since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. gGBEv6.3-SpCas9

    Plasmid

    #202629

    Purpose

    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

    Insert

    nSpCas9(D10A)-MPGv6.3
    

    Expression

    Mammalian

    Mutation

    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

    Promoter

    hU6, EF1a, CBh

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-SMARCA4

    Plasmid

    #65391

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA

    Plasmid

    #171383

    Purpose

    Expression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized minidCas13X.1-REPAIRv2
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV, U6

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiGuide Blast

    Plasmid

    #199622

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

    Plasmid

    #168305

    Purpose

    mediated knock-in of sgRNA precursor

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    SaKKH-EGFP-U6-sgRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1alpha, CBh

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

    Plasmid

    #208959

    Purpose

    A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)

    Plasmid

    #208954

    Purpose

    A variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A), Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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