We narrowed to 9,980 results for: Uty

1. AWP-029

   Plasmid

   #213966

   Purpose

   Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.

   Depositor

   Alexander Westermann

   Article

   Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

   Insert

   Cas12a (cas12a Segatella bryantii, Synthetic)
   

   Tags

   3XFLAG

   Expression

   Bacterial

   Mutation

   D875A to inactivate target nuclease activity

   Promoter

   PcfxA, IPTG inducible

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV7.1_3xFLAG-TAOK1

   Plasmid

   #172991

   Purpose

   constitutive expression of FLAG-tagged TAOK1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-TAOK1 (TAOK1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. tetO-FUW-eGFP-RHOA-Q63L

   Plasmid

   #73081

   Purpose

   Lentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-Q63L

   Depositor

   Andrew Putnam

   Article

   Kong et al Sci Rep. 2013 Dec 11;3:3474. doi: 10.103

   Insert

   RHOA-Q63L (RHOA Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Q63L Constitutively active

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. hCMV-IE1:RedF

   Plasmid

   #118064

   Purpose

   Transcriptional unit for constitutive expression of RedF in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:RedF:bGHF
   

   Use

   Luciferase and Synthetic Biology; Assembly of gb2…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-MLC2(CA)-IRES-GFP

   Plasmid

   #133928

   Purpose

   Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   EGFP and MLC2(CA) (MYL2 Human)
   

   Expression

   Mammalian

   Mutation

   MLC2 T18D, S19D

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDule-para-aminoPhe

   Plasmid

   #85502

   Purpose

   Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

   Insert

   p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32T, E107T, D158P, I159L, L162A

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-20b-M-Ntx-S1

   Plasmid

   #58716

   Purpose

   Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Changed Asparagine 1 to Serine

   Promoter

   T7

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBLADE(FP6*)-mCherry

   Plasmid

   #168048

   Purpose

   Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

   Depositor

   Barbara Di Ventura

   Article

   Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

   Inserts

   Blue light-inducible AraC dimers in Escherichia coli
   mCherry
   

   Expression

   Bacterial

   Promoter

   J23101* and PBAD

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5E-CAGGS (JDW 912)

   Plasmid

   #224472

   Purpose

   Gateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   CAGGS Promoter
   

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPS0382

    Plasmid

    #133232

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3015b

    Plasmid

    #209317

    Purpose

    Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

    Depositor

    Thomas Hooven

    Article

    Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

    Insert

    Group B Streptococcus-compatible single guide RNA
    

    Expression

    Bacterial

    Promoter

    Xyl/tet promoter that is constitutively active

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRE-Tn5-133

    Plasmid

    #118519

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV7.1_3xFLAG-TAOK2

    Plasmid

    #172992

    Purpose

    constitutive expression of FLAG-tagged TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK2 (TAOK2 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. hCMV-IE1:GrRenilla

    Plasmid

    #118067

    Purpose

    Transcriptional unit for constitutive expression of GrRenilla in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:GreenRenilla:bGHF
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. SSTR3-DuET

    Plasmid

    #213378

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR3 (SSTR3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRE-Tn5-135

    Plasmid

    #118521

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTorPE-R-GECO1

    Plasmid

    #32465

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Lab constructed

    Tags

    6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    Substitutions relative to the mApple-derived anal…

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. OPN3-DuET

    Plasmid

    #213357

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPN3 (OPN3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5 TGF beta type I receptor (T202D) Flag

    Plasmid

    #14833

    Depositor

    Rik Derynck

    Article

    Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

    Insert

    TGF beta type I receptor (Tgfbr1 Rat)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Threonine 202 mutated to aspartate. Constitutivel…

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. meffBlue chromoprotein

    Plasmid

    #117845

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, meffBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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