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AB.pCCL.sin.cPPT.U6.miR-181b-Decoy.hPGK.GFP.WPRE

Plasmid

#46595

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-181b Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
mCerulean3-PTK2-N-10

Plasmid

#55442

Purpose

Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

PTK2 (PTK2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
TAL2314

Plasmid

#36740

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCF-TALEN_Left (Fancf Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
TAL2416

Plasmid

#36817

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCA-TALEN_Left (Fanca Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
mEos2-Caveolin-C-10

Plasmid

#57355

Purpose

Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Caveolin (CAV1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pHH0103_PPARG_Hormone-recep

Plasmid

#109956

Purpose

Protein expression and purification of PPARG_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PPARG_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mEos2-Actin-C-18

Plasmid

#57340

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Actin (ACTB Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Nano-lantern(Ca2+)_11/pRSETB

Plasmid

#51981

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-4GS

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern(cAMP-3.3)/pRSETB

Plasmid

#53592

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Tags

6xHis

Expression

Bacterial

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pTrcHis-7D12CTermTAG

Plasmid

#125267

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at c-terminus for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

CTermTAG

Tags

His-Tag

Promoter

Trc

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTrcHis-7D12Gly42TAG

Plasmid

#125265

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 Gly42 nanobody

Tags

His-tag

Expression

Bacterial

Promoter

trc promoter

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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TAL2400

Plasmid

#36803

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HDAC6-TALEN_Left (Hdac6 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2296

Plasmid

#36729

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BAX-TALEN_Left (Bax Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pDGE658

Plasmid

#153256

Purpose

M3 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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TAL2328

Plasmid

#36754

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MLH1-TALEN_Left (Mlh1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-del 50-59

Plasmid

#33065

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 50-59

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pHH0103_NR1I3_Hormone-recep

Plasmid

#109944

Purpose

Protein expression and purification of NR1I3_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR1I3_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR4A1_Hormone-recep

Plasmid

#109951

Purpose

Protein expression and purification of NR4A1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR4A1_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CT-Nano-lantern(ATP1)/pENTR1A

Plasmid

#51991

Purpose

Gateway entry vector for Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Use

Entry vector

Tags

chloroplast transit peptid

Mutation

S257G in Rluc8

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
JBEI-6823

Plasmid

#84986

Purpose

For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-PMKco-Mkco

Expression

Bacterial

Promoter

LacUV5

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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