We narrowed to 13,278 results for: sequence
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Plasmid#71719PurposeExpressing a GFP-tagged degron in the soma of C. elegansDepositorInsertauxin-responsive protein IAA17 (AXR3 Mustard Weed)
TagsEmGFPExpressionWormMutationminimal degron sequence (71-114aa) with start cod…Promotereef-1A.1 (eft-3)Available SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-SialT
Plasmid#87324PurposeTo express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferaseDepositorInsertST 6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1 Human)
TagsAcGFP1ExpressionMammalianPromoterCMVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tom70 (1-70)-GFP
Plasmid#127633PurposeMitochondrial targeting sequence of Tom70 fused to GFPDepositorAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDS240
Plasmid#34966PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastMutationK533∆, high-affinity epitopePromoterGAL1Available SinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeA
Plasmid#128943Purposeexpresses PfeA in E. coliDepositorInsertferric enterobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75)PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A
Plasmid#110555PurposepFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.DepositorInsertAnti-RD114 heavy chain
TagsmIgG2AExpressionBacterial and MammalianPromoterEF-1 alpha core promoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBUN421
Plasmid#62204PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterTaU3 and UbiAvailable SinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19/N-Vc
Plasmid#183157PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-BoxB
Plasmid#92004PurposeExpresses firefly luciferase with BoxB sequences in 3' UTRDepositorInsertFirefly Luciferase
UseLuciferaseExpressionMammalianAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mucolipin1-pEGFP C3
Plasmid#62960Purposefull length human Mucolipin-1 cloned into pEGFP C3DepositorInsertMucolipin-1 (MCOLN1 Human)
TagsEGFPExpressionMammalianMutationMucolipin-1 has 1 silent mutation compared to the…PromoterCMVAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSP6-bio
Plasmid#47732PurposeExpresses enzymatically monobiotinylated full-length MSP6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP6
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MP28445 – pFUSE2ss-aRD114_CLIg-mIgK
Plasmid#110556PurposepFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.DepositorInsertanit-RD114 light chain
TagsmKappaExpressionBacterial and MammalianPromoterEF-1 alpha core promoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSP11-bio
Plasmid#47734PurposeExpresses enzymatically monobiotinylated full-length MSP11 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP11
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
AARP-bio
Plasmid#47783PurposeExpresses enzymatically monobiotinylated full-length AARP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised AARP
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSP4-bio
Plasmid#47717PurposeExpresses enzymatically monobiotinylated full-length MSP4 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP4
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-pAceR-Kv2.1PR
Plasmid#195534PurposeCre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterCAGAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Vc-C
Plasmid#183158PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)
Plasmid#70191PurposeDerived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.DepositorInsertClpX (clpX )
Available SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLBPVXBa-M
Plasmid#229079PurposeNew version of a PVX vector to express heterologous sequences in plantsDepositorInsertPotato virus X
ExpressionPlantMutationMutation coat protein start codon (ATG to AGG); d…Available SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYL322d1/N-Vn
Plasmid#183153PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only