We narrowed to 13,677 results for: FLU;
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Plasmid#178967PurposeExpresses miRFP2 and HO1 in mammalian cellsDepositorInsertmiRFP2
TagsHO1 and noExpressionMammalianMutationNOPromoterCMVAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1043
Plasmid#169621PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .DepositorInsertPCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1042
Plasmid#169620PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(K468TAG)-FLAG
Plasmid#182658PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K468 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
TagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK468TAGPromoterCMVAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(K211TAG)-FLAG
Plasmid#182655PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K211 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)
TagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK211TAGPromoterCMVAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(R438TAG)-FLAG
Plasmid#182657PurposeExpresses mouse neurofilament light chain with a TAG codon at the position R438 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a R438TAG mutation (Nefl Mouse)
TagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationR438TAGPromoterCMVAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_NFL linker-3xFLAG KI
Plasmid#182679PurposeExpression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.DepositorInsertNefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
ExpressionMammalianPromoterU6 and chicken beta-actin promoterAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_NFL linker(A6TAG)-3xFLAG KI
Plasmid#182680PurposeExpression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.DepositorInsertNefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
ExpressionMammalianPromoterU6 and chicken beta-actin promoterAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_FLAG-NFL(K468TAG)
Plasmid#182663PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K468 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
TagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK468TAGPromoterCMVAvailable SinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPOLY_new
Plasmid#179276PurposeExpresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)DepositorInsertscellodextrin phosphorylase
cellobiose phosphorylase
sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
UseSynthetic BiologyTags6xHIS and Strep-Tag IIExpressionBacterialPromoterT7 lacOAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBICI_high ori
Plasmid#179273PurposeExpresses CuCBP and BaSP in E. coli BL21(DE3)DepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialPromoterT7 lacOAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDUBI
Plasmid#179274PurposeExpresses CcCDP monocistronically and CuCBP and BaSP bicistronicallyDepositorInsertscellodextrin phosphorylase
cellobiose phosphorylase
C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
UseSynthetic BiologyTags6xHIS and Strep-Tag IIExpressionBacterialMutationsucrose phosphorylasePromoterT7 lacOAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG660 lyn11-pFAST
Plasmid#172863Purposeexpresses lyn11-pFAST in mammalian cells (inner membrane labeling)DepositorInsertLyn11-pFAST
TagsMyc tag (C terminal on insert)ExpressionMammalianPromoterCMVAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT3038
Plasmid#122545PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal related organelle localized gene (glo-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3043
Plasmid#122546PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 RR837-8GG
Plasmid#113958Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-839, R837G and R838G substitutionPromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-9-3p
Plasmid#103748PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-9-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-9-3p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-92a-3p
Plasmid#103752PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-92a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-92a-3p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-548h-3p
Plasmid#103635PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-548h-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-548h-3p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-548h-5p
Plasmid#103636PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-548h-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-548h-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only