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NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75238

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75239

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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FHp5pUP95C3,5SGW (E5)

Plasmid

#74035

Purpose

lentiviral expression of Psd95 shRNA and Psd95-C3,5S-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Publication

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

PCR: C3,5S (tct aga cca cca tgg act Ctc tAt CGa t…

Promoter

H1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
mPapaya-Farnesyl-5

Plasmid

#56643

Purpose

Localization: Membrane, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Publication

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Farnesyl (HRAS Human)

Tags

mPapaya

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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VV228: sdChR(C138S E154A)-TS in fck

Plasmid

#58523

Purpose

Expresses sdChR(C138S E154A) in mammalian cells

Depositor

Adam Cohen

Publication

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S, E154A)-TS

Use

Lentiviral

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine; changed Glutamate…

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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hSRAP.TWIN1.a13-69

Plasmid

#49815

Purpose

expression vector for SRAP(13-69) in pTWIN1 vector

Depositor

Thomas Cech

Publication

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-69 (SRA1 Human)

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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hSRAP.TWIN1.a13-87

Plasmid

#49816

Purpose

expression plasmid for SRAP(13-87) in pTWIN1 vector

Depositor

Thomas Cech

Publication

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-87 (SRA1 Human)

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pMALp2x-SCO5461(43-204)

Plasmid

#42509

Depositor

Koji Okamoto

Publication

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Tags

maltose-binding protein

Expression

Bacterial

Mutation

deleted amino acids 1-42 (nucleotide 1-126)

Promoter

tac

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pRSET FLIPE intramol-10u

Plasmid

#13550

Depositor

Wolf Frommer

Publication

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol A207R (gltI E. coli)

Tags

EYFP

Expression

Bacterial

Mutation

A207R, ECFP insert in binding protein

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Cx35-M1-FLAG-T2A-mCherry

Plasmid

#255638

Purpose

Encodes floxed (DIO) M. americana Cx35, codon optimized for mouse expression and c-terminally fused FLAG tag, T2A self-cleaving peptide and mCherry. Mutation of K221E. hSyn promoter.

Depositor

Kafui Dzirasa

Publication

Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586

Insert

Cx35

Use

AAV

Tags

FLAG-T2A-mCherry

Mutation

K221E

Promoter

hSyn

Available since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-P65-HSF1

Plasmid

#218559

Purpose

Encodes SFFV promoter-driven MCP-P65-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Publication

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-P65-HSF1

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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EK0007-PB-4XnrUAS-H2B-BFP

Plasmid

#236121

Purpose

Fuorescent reporter encoding H2B-tagBFP fusion under control of E1b minimal promoter with four Gal4-binding sites upstream

Depositor

Xiaojing Gao

Publication

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B (H2BC11 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

E1b minimal promoter with four Gal4-binding sites…

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW410 IKZF1tar x8-H2B-GFP

Plasmid

#236156

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight IKZF1 binding sites upstream

Depositor

Xiaojing Gao

Publication

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with eight IKZF1 binding sit…

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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PX459-CTNNA1(α-catenin CRISPR KO)

Plasmid

#229708

Purpose

Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology

Depositor

Alpha Yap

Publication

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Expression

Mammalian

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CROPseq-Puro-12xMS2

Plasmid

#212628

Purpose

MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

Depositor

Paul Blainey

Publication

Najia et al Cell. 2026 Sep 1:S0092-8674(26)00932-3.

Insert

EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHR RPS27A K107/113R-3xFlag_IRES-GFP

Plasmid

#198392

Purpose

RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Publication

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

ubiquitination site double mutant (K107/113R)

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR3DX1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156774

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR3DX1 (KIR3DX1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTR-341 pUC19-tNGFR-P2A-PDCD1 N

Plasmid

#186074

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Publication

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PDCD1 (PDCD1 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEF086 MBP-CREB 127-135-His

Plasmid

#154075

Purpose

Expresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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px459-Rheb sgRNA

Plasmid

#133768

Purpose

Expresses Cas9 and human Rheb sgRNA

Depositor

Shawn Ferguson

Publication

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rheb sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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