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pHIE056 ZF43x6-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138737

Purpose

Integration Vector, assembled with pHIE048 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE057 ZF43x12-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138738

Purpose

Integration Vector, assembled with pHIE049 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE054 ZF43x12-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138735

Purpose

Integration Vector, assembled with pHIE046 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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R619-M68-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP

Plasmid

#166019

Purpose

mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S )

Tags

3C-His8-SBP

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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R701-X56-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D

Plasmid

#159564

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G13D

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X22-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D

Plasmid

#159571

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G13D

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X53-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C

Plasmid

#159561

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12C

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105210

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6xHis-thrombin-SUMO-HsTNRC6B(599-683)-3xHA

Plasmid

#254374

Purpose

Expression of TNRC6B fragment for protein purification

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human TNRC6B (TNRC6B Human)

Tags

3xHA and 6xHis-thrombin-SUMO

Expression

Bacterial

Mutation

Fragment consisting of residues 599-683

Promoter

T7

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pET28a_6xHis-thrombin-SUMO-HsTNRC6B(599-683)-3xFLAG

Plasmid

#254375

Purpose

Expression of TNRC6B fragment for protein purification

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human TNRC6B (TNRC6B Human)

Tags

3xFLAG and 6xHis-thrombin-SUMO

Expression

Bacterial

Mutation

Fragment consisting of residues 599-683

Promoter

T7

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pET156P CFAP36 [W10E]-3C-muGFP-10His (Sus scrofa)

Plasmid

#238219

Purpose

Bacterial expression vector of porcine CFAP36 W10E mutant with C-terminal tags

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [W10E]-3C-muGFP-10His

Tags

-3C-muGFP-10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pET156P CFAP36 [S92E]-3C-muGFP-10His (Sus scrofa)

Plasmid

#238220

Purpose

Bacterial expression vector of porcine CFAP36 S92E mutant with C-terminal tags

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [S92E]-3C-muGFP-10His

Tags

-3C-muGFP-10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pET156P CFAP36 [A123E]-3C-muGFP-10His (Sus scrofa)

Plasmid

#238221

Purpose

Bacterial expression vector of porcine CFAP36 A123E mutant with C-terminal tags

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [A123E]-3C-muGFP-10His

Tags

-3C-muGFP-10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pET156P CFAP36 [S163E]-3C-muGFP-10His (Sus scrofa)

Plasmid

#238222

Purpose

Bacterial expression vector of porcine CFAP36 S163E mutant with C-terminal tags

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [S163E]-3C-muGFP-10His

Tags

-3C-muGFP-10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pET156P CFAP36 [S180E]-3C-muGFP-10His (Sus scrofa)

Plasmid

#238223

Purpose

Bacterial expression vector of porcine CFAP36 S180E mutant with C-terminal tags

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [S180E]-3C-muGFP-10His

Tags

-3C-muGFP-10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet_6xHis-TEV-mCherry-ATG13 IDR(230-517aa)

Plasmid

#227711

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

mCherry-ATG13 IDR(230-517aa) (ATG13 Human)

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-WIPI2d IDR (364-425aa)

Plasmid

#223790

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2d IDR (WIPI2 Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Mutation

aa 364-425 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa)

Plasmid

#223762

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Plasmid

#200792

Purpose

Mammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

Δ954-1027

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Plasmid

#200798

Purpose

Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

411-680

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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