We narrowed to 11,803 results for: nsf

1. pBEG R2-iHygro-L3 #BGV206

   Plasmid

   #48985

   Purpose

   Supplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   hygromycin phosphotransferase
   

   Use

   Gateway cloning vector

   Tags

   IRES (weak)

   Promoter

   na

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

   Plasmid

   #78606

   Purpose

   pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2
   

   Use

   AAV and CRISPR

   Tags

   HA tag

   Expression

   Mammalian

   Promoter

   173CMV, U6

   Available Since

   Dec. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE

   Plasmid

   #120269

   Purpose

   Allows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent protein

   Depositor

   Edward Callaway

   Article

   Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

   Insert

   eSyn-DIO-TVA950:YFP
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-WPRE

   Plasmid

   #127864

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Promoter

   hSyn

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-dCas9-MQ1(S317A)-EGFP

   Plasmid

   #89636

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG and 6*His

   Expression

   Mammalian

   Mutation

   S317A, AGC-->GCC

   Promoter

   CMV

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENN.AAV.CB7.CI.CNDP1-flag.WPRE.rBG

   Plasmid

   #132683

   Purpose

   AAV plasmid encoding mouse CNDP1 with C-terminal flag tag

   Depositor

   Jonathan Long

   Article

   Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

   Insert

   Carnosine dipeptidase 1 (Cndp1 Mouse)
   

   Use

   AAV

   Tags

   Flag

   Promoter

   Chicken b-actin

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. tdTomato/pTREX-b

   Plasmid

   #68709

   Purpose

   Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Insert

   tdTomato
   

   Use

   Expression of tdtomato red fluorescence protein i…

   Available Since

   Oct. 8, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV-CAG-DIO-CFL-SN-P2A-GCaMP6f

   Plasmid

   #181742

   Purpose

   AAV vector for expressing CFL-SN and GCaMP6f

   Depositor

   Yasunori Hayashi

   Article

   Goto et al Science. 2021 Nov 12;374(6569):857-863.

   Insert

   CFL-SN-P2A-GCaMP6f
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Htr5b(3.7)-EGFP-sv40

   Plasmid

   #184411

   Purpose

   AAV expression of EGFP from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

   Depositor

   Marylka Yoe Uusisaari

   Article

   Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

   Insert

   Mouse Htr5b gene promoter-EGFP (Htr5b Mouse)
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Mouse Htr5b gene

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-IRES-hrGFP-mWnt5a

    Plasmid

    #89772

    Purpose

    Expresses mouse Wnt5a in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Rodriguez et al J Neurosci. 2014 Jul 30;34(31):10285-97.

    Insert

    mouse Wnt5a (Wnt5a Mouse)
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pM124: pAAV-EFS-CasRx-VEGFA presgRNA

    Plasmid

    #166872

    Purpose

    AAV vector for expressing CasRx and human VEGFA targeting presgRNA for RNA-editing

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Inserts

    U6-VEGFA presgRNA
    RfxCas13d
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. All-in-one split-ADAR2(E488Q, N496F)

    Plasmid

    #170148

    Purpose

    AAV vector carrying ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS

    Plasmid

    #199355

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA-SUMOstar

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CMV.VTN.C-HA

    Plasmid

    #154849

    Purpose

    Expresses murine Vitronectin (VTN) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Vitronectin (Vtn Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-eGFP-SynaptoZip

    Plasmid

    #122525

    Purpose

    AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

    Depositor

    Antonio Malgaroli

    Article

    Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

    Insert

    eGFP-SynaptoZip (Vamp2 Rat)
    

    Use

    AAV

    Tags

    Myc and eGFP

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMflET-o4

    Plasmid

    #101314

    Purpose

    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Inserts

    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    ereB resistance gene
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

    Plasmid

    #186991

    Purpose

    AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    cas7-11-r1045-gggs-r1122, non-targeting crRNA guide
    

    Use

    AAV

    Mutation

    r1045-gggs-r1122 deletion

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-IFITM3-F8-TAG

    Plasmid

    #122475

    Purpose

    expresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Phenylalanine 8 to amber codon

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTS112 pHAGE2 CMVtetO2 MCS-TEV-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199365

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV_Flex_syn_SpikeyGi2_kv2.1

    Plasmid

    #195314

    Purpose

    Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, double-flowed in AAV vector under the promoter hSyn

    Depositor

    Vincent Pieribone

    Article

    Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

    Insert

    SpikeyGi2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human synapsin promoter

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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