We narrowed to 36,704 results for: ELL

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pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2

Plasmid

#113889

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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RAB11A-mCherry HDRT Source (pTR 144)

Plasmid

#112013

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-mCherry HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pOBCol2.3-GFPemd

Plasmid

#110210

Purpose

Labels cells in the late osteoblast lineage.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPemerald

Tags

None

Expression

Mammalian

Promoter

2.3 kb rat type 1 collagen + first intron

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pInducer20-Blast-CDT1-HA

Plasmid

#109335

Purpose

virus packaged, doxycyline inducible expression of Cdt1

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

Cdt1 (CDT1 Human)

Use

Lentiviral; Inducible expression

Tags

HA, His, Strep

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 SV40-CMV-SaCas9-3xNLS

Plasmid

#78601

Purpose

AAV vector containing SaCas9

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

SV40-CMV-SaCas9-3xNLS

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

SV40, CMV promoters

Available Since

Dec. 2, 2017

Availability

Academic Institutions and Nonprofits only
pmCherry-MYRIP

Plasmid

#89586

Purpose

Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYRIP (Myrip Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(E17K)

Plasmid

#86563

Purpose

Expresses human full-length Akt1(E17K) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

E17K mutation in the PH domain

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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PCXLE-hUL + mTAGBFP2

Plasmid

#74944

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

LMyc/Lin28 (MYCL, LIN28A Human)

Expression

Mammalian

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-hChR2-YFP

Plasmid

#73465

Purpose

Expresses channelrhodopsin fused to YFP for monosynaptic circuit mapping and optogenetic activation

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

hChR2-YFP

Use

Neurotropic virus

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pUAST-GFP-RpL10Ab

Plasmid

#71729

Purpose

Expression of Drosophila melanogaster Rpl10Ab-GFP fusion

Depositor

Herman Dierick

Article

Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

Insert

RpL10Ab (RpL10Ab Fly)

Tags

EGFP

Expression

Insect

Promoter

hsp70

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA-FRT-FAK10

Plasmid

#59119

Purpose

FAK fret sensor with 10 nm helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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NpT7-5-ERK1

Plasmid

#39229

Depositor

Melanie Cobb

Article

Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

Insert

ERK1 (MAPK3 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
FL-BCAN-lentiviral-GFP

Plasmid

#29704

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

Brevican (BCAN Human)

Use

Lentiviral

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pSuper mouse Drosha3

Plasmid

#14776

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (Drosha Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
JD150

Plasmid

#229485

Purpose

Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E2A, VA RNA I, E4orf6, L4-22k/33k

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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RNA-Protein Landing Pad

Plasmid

#226162

Purpose

Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Inserts

mTagBFP2-P2A-iCasp9
Reverse tetracycline transactivator 3
Hygromycin-IRES-EGFP

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR

Plasmid

#213480

Purpose

AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mtagBFP-KASH
U6-gRNA-directcapture

Use

AAV

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-Fluc-PGK-Rluc-mCherry

Plasmid

#191863

Purpose

Heat inducible Fluc reporter with constitutive Rluc

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

Firefly luciferase Fluc
Renilla luciferase Rluc

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Promoter

HSPA7 promoter and PGK promoter

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_YBX3-Halo

Plasmid

#175308

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

YBX3 (YBX3 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pet-15b-microID

Plasmid

#172881

Purpose

expresses microID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

microID

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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