We narrowed to 9,980 results for: Uty

1. XW17

   Plasmid

   #65835

   Purpose

   Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)

   Depositor

   Kang Shen

   Article

   Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

   Insert

   OR74A quinic acid utilization activator (qa-1F)
   

   Tags

   mCherry

   Expression

   Worm

   Mutation

   The endogenous SphI, KpnI and SacI sites in QF ar…

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJM529 EF1α 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA in TUPV5

   Plasmid

   #161555

   Purpose

   Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG and HA

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLIK-Neo-TmiR-Luc

   Plasmid

   #25743

   Purpose

   Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Neomycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Luciferase miR-shRNA
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCXLE-dCas9VPP300-T2A-GFP-shP53

   Plasmid

   #102896

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9VPP300-T2A-GFP-shP53
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPS0380

   Plasmid

   #133230

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRE-Tn5-144

   Plasmid

   #118528

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mOrange2
   

   Use

   Minitn5 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_nV5-TAOK2

   Plasmid

   #173000

   Purpose

   constitutive expression of V5-tagged TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   V5-TAOK2 (TAOK2 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBlastR-5xUAS-sfGFP-MiniWhite

   Plasmid

   #165910

   Purpose

   Blasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable UAS Response Vector
   

   Use

   Synthetic Biology

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB1C3_YPet-PCP (y-plasmid)

   Plasmid

   #127637

   Purpose

   Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

   Insert

   YPet
   

   Use

   Synthetic Biology

   Tags

   RNA binding peptide: PP7 coat protein (PCP)

   Expression

   Bacterial

   Promoter

   pJ21306

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1

    Plasmid

    #56658

    Purpose

    expresses constitutively active human PKCi with a deletion on the PB1 domain

    Depositor

    Pedro Salas

    Article

    Forteza et al Biol Open. 2013 Oct 8;2(11):1264-9. doi:

    Insert

    Human PKCi (PRKCI Human)
    

    Use

    Lentiviral

    Tags

    V5/His

    Mutation

    A120E and deletion of PB1 domain (aa 24–98)

    Promoter

    CMV

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

11. DT034A

    Plasmid

    #159395

    Purpose

    Constitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
    

    Use

    Synthetic Biology

    Tags

    FLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…

    Expression

    Bacterial

    Promoter

    J23117 and pLux promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PhyB-mCherry-MYO1

    Plasmid

    #51570

    Purpose

    Constitutively express myosin ring localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    MYO1 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. SIN40C.SFFV.mTagBFP2.let-7c

    Plasmid

    #169310

    Purpose

    Constitutive overexpression of let-7c with mTagBFP2 as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    let-7c (MIRLET7C Human)
    

    Use

    Lentiviral

    Tags

    TagBFP2

    Promoter

    SFFV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. SIN40C.SFFV.dTomato.miR-125b-2

    Plasmid

    #169302

    Purpose

    Constitutive overexpression of miR-125b-2 with dTomato as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    miR-125b-2 (MIR125B2 Human)
    

    Use

    Lentiviral

    Tags

    dTomato

    Promoter

    SFFV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p35S::IRE1a

    Plasmid

    #135232

    Purpose

    constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    IRE1a (IRE1A Mustard Weed)
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBlastR-5xUAS-mCherry-MiniWhite

    Plasmid

    #165911

    Purpose

    Blasticidin resistant 5xUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. DT050A

    Plasmid

    #159411

    Purpose

    Constitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23101-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    J23101 promoter

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. SIN40C.SFFV.GFP.miR-99a

    Plasmid

    #169304

    Purpose

    Constitutive overexpression of miR-99a with GFP as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    miR-99a (MIR99A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    SFFV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST

    Plasmid

    #175018

    Purpose

    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

    Insert

    OK-HEF s57a ED
    

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    s57a esterase knock out mutant

    Promoter

    CMV

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBlastR-12xLexAOp-EBFP2-MiniWhite

    Plasmid

    #165918

    Purpose

    Blasticidin resistant 12xLexAOp EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable LexAOp Response Vector
    

    Use

    Synthetic Biology

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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