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puC19 preACT1(WT)

Plasmid

#111323

Purpose

for T7 transcription of wt actin pre-mRNA

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pWT055h

Plasmid

#96867

Purpose

sgRNA-HEK4

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-HEK4

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCG-A-sacB(SP)-B

Plasmid

#87370

Purpose

An ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SacB signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET11b_MutT

Plasmid

#73155

Purpose

Expresses in E. coli, E coli MutT

Depositor

Maurice Bessman, Sandra Gabelli

Article

Bessman et al J Biol Chem. 1996 Oct 11;271(41):25059-6

Insert

MutT

Expression

Bacterial

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
pST50Trc4-CBPHISNDHFR

Plasmid

#63999

Purpose

Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

Depositor

Song Tan

Article

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Tags

6xHis & Calmodulin Binding Peptide (CBP); TEV…

Expression

Bacterial

Promoter

T7

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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Phamret/pcDNA3

Plasmid

#51953

Purpose

mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein.

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

Phamret

Expression

Mammalian

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pAraTM-2BTMCYL980A

Plasmid

#39550

Depositor

Bryan Berger

Article

Su et al J Biol Chem. 2012 Jul 22.

Insert

Integrin alpha2B L980A TM-CYTO

Expression

Bacterial

Promoter

Tac

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pRSF-KpDcr1deltaC

Plasmid

#32023

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Tags

His6-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pHZ149

Plasmid

#27509

Depositor

Andrew Fire

Article

Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

Insert

pREAR::DsRed::unc-54_3'UTR

Expression

Worm

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pEM403

Plasmid

#248736

Purpose

pLacI43 driving sfGFP in pNBU2 integrative Bacteroides plasmid

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

PBfP2E5-RBS1PLP-LacI-rrnBT1-pLacI43-RBS1PLP-sfGFP-rrnBT2

Expression

Bacterial

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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anti-GCN4_scFv_GFP

Plasmid

#244061

Purpose

fusion of the SunTag binder (anti GCN4 scFv) to sfGFP

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

anti-GCN4_scFv_sfGFP_GB1

Expression

Mammalian

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-TBD

Plasmid

#236737

Purpose

Protein overexpression of Thalidomide Binding domain of hCRBN

Depositor

Christina Woo

Article

Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

Insert

Thalidomide Binding domain of hCRBN (CRBN Human)

Tags

His6

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Immunostealth

Plasmid

#239446

Purpose

Expresses murine NIS (mNIS) in mammalian cells for non-immunogenic in vivo imaging using SPECT or PET

Depositor

Scott Lyons

Article

Merrill et al Cell Stress. 2023 Aug 14;7(8):59-68. doi

Insert

sodium iodide symporter (Slc5a5 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-AP-Ub-dGG

Plasmid

#232581

Purpose

Express Acceptor Peptide (AP) tagged Ubiquitin lacking the two terminal glycines

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AviTag

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL3-01.EGFP.BCA5.WPRE

Plasmid

#225685

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-01.EGFP.BCA5

Expression

Mammalian

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pTW357b

Plasmid

#227930

Purpose

Low-stringency accessory plasmid for selection

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Inserts

gIII (recoded)
T7c

Expression

Bacterial

Mutation

R632S

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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EF1m-ELP

Plasmid

#170763

Purpose

EF1m laminin α1 peptide fused to I30-ELP (VPGIG)30

Depositor

Andrew MacKay

Article

Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

Insert

EF1m-ELP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pGL0_7 [pSa ORI]

Plasmid

#198923

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSa ORI

Use

Synthetic Biology

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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Sha2Cas9

Plasmid

#192140

Purpose

Expresses Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBbE2k-pTet-gRNAwcaF159

Plasmid

#122259

Purpose

aTc inducible gRNA expression plasmid

Depositor

Chueh Loo Poh

Article

Zhang et al Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.

Insert

gRNAwcaF159

Use

Synthetic Biology

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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