We narrowed to 16,981 results for: tes
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Plasmid#219396PurposeExpress HNF4A in mammalian cellsDepositorAvailable SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only
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pTRIPZ-p21-HA(RxL)
Plasmid#215111PurposeExpresses p21-HA(RxL) in mammalian cells from an inducible lentiviral vector.DepositorInsertCDKN1A (CDKN1A Human)
UseLentiviralTagsHAExpressionMammalianMutationp21(RxL) denotes mutations: R19A, R20A, L21A, F22…PromoterCMV (Tet inducible)Available SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-bioGEF-SUMO1nc-PURO
Plasmid#208042PurposeEnables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycinDepositorInsertSUMO1nc (SUMO1 Human)
UseLentiviralTagsLow-affinity AvitagMutationQ94P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-mCherry-Rheb(C181S)
Plasmid#192438PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.DepositorInsertLyso-mCherry-Rheb(C181S) (RHEB Human)
TagsFull-length LAMP1 and mCherryExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(C181S)
Plasmid#192437PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSN840
Plasmid#184832PurposeExpresses human KIF5A(∆exon27) and human KLC1 in insect cellsDepositorUseCre/LoxTagsHis-FLAG and mScarlet-2xStrepIIExpressionInsectMutation∆exon27 form of human KIF5APromoterp10 and polyhedrinAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTV001
Plasmid#130265PurposeEatA expression plasmid containing 8His encoding region in permissive site of eatA geneDepositorInserteatA::His8
Tagspolyhistidine (internal)ExpressionBacterialMutation8His polyhistidine encoding region and XhoI site …PromoteraraBADAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
SOX2 KO (T2A-H2B-EGFP)
Plasmid#167969PurposeSOX2 CDS replacement with T2A-H2B-EGFPDepositorInsertSRY-Box Transcription Factor 2 (SOX2 Human)
UseCRISPR; Donor templateTagsH2B-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC71167
Plasmid#154066PurposeLevel 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFPDepositorInsertZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
UseSynthetic BiologyMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterZea mays Ubiquitin PromoterAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP
Plasmid#153071PurposeLentiviral vector expressing human ZC3H12D mutated (D95N)DepositorInsertMutated ZC3H12D (D95N) (ZC3H12D Human)
UseLentiviralExpressionMammalianMutationChanged D95 to N, known to abrogate the RNase enz…PromoterEF1αAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgACSL3.C-Cas9-P2A-Puro
Plasmid#129412PurposeEncoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminusDepositorInsertSpCas9 and sgACSL3.C (ACSL3 Human)
UseCRISPRTagsP2A-PuroExpressionMammalianPromoterhU6Available SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-U6-shHMGA2.2-PGK-dTomato
Plasmid#89605PurposeSilencing Hmga2 in human cellsDepositorAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-YOD1-MutS2
Plasmid#85667PurposeExpression of human YOD1 I292Q V295Q mutant with N-terminal GFP tagDepositorAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
XE250 pCDNA3.1 + (zeo)- VSV-KLHL12-Q405X
Plasmid#16759DepositorAvailable SinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV T274V S392A KSR1 IRES GFP
Plasmid#25979DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationchanged threonine 274 to valine and serine 392 to…Available SinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-flex-iGluSnFR4f-PDGFR-WPRE
Plasmid#234440PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4f-PDGFR
UseAAV and Cre/LoxTagsIgK chain and Myc epi tag-PDGFR TM DomainExpressionMammalianPromoterSynapsinAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Tnnt2-GFP-LA
Plasmid#206198PurposeExpresses GFP-tagged Lamin-A specifically in cardiomyocytes by the Tnnt2 promoterDepositorAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA S27D
Plasmid#139194PurposeExpresses C-terminal HA tagged human Mfn2 S27D in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
Tags2HAExpressionMammalianMutationSerine 27 to Aspartic AcidPromoterCMV promoterAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRM053 His-TEV-Cas9 triNLS 0C
Plasmid#196244PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-BS-AtMIR390a-B/c
Plasmid#199560PurposePlant expression vector (2x35S) for direct cloning of amiRNAs into Arabidopsis thaliana MIR390a precursor including only the basal stemDepositorInsertBasal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)
UseRNAiPromoter2x35SAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only