Addgene

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pnYC-NpH-SceIF4E_25-213-K42AK168AK187A_AF

Plasmid

#148728

Purpose

Bacterial Expression of SceIF4E_25-213-K42AK168AK187A

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

SceIF4E_25-213-K42AK168AK187A (CDC33 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 187-dendra2

Plasmid

#97117

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 187 dendra2

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 N187-207 (#596)

Plasmid

#99940

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delAA187-207insNAAIRS linker

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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3187 pSFFV-neo Bad cDNA ORF

Plasmid

#8756

Depositor

Stanley Korsmeyer

Article

Zha et al Cell 1996 Nov 15;87(4):619-28.

Insert

BAD (Bad Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pMXS-IRES-BLAST flag-CAD R2187A

Plasmid

#169827

Purpose

Expresses C-terminal flag-tagged CAD with mutation at reported trimerization interface of the ATCase domain of CAD

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

R2187A, T456A S1406A S1859A; TCCC -> AGTC sile…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-FAM187A-COMP5AP-AviTag-9xHis

Plasmid

#157366

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

FAM187A (FAM187A Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-FAM187A-Fc(DAPA)-AviTag-6xHis

Plasmid

#156802

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

FAM187A (FAM187A Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1874 - pAAV-AiE2397m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230726

Purpose

AiE2397m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1875 - pAAV-AiE2403m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230727

Purpose

AiE2403m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1877 - pAAV-AiE2423m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230728

Purpose

AiE2423m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1870 - pAAV-AiE2492m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230724

Purpose

AiE2492m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1872 - pAAV-AiE2517m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230725

Purpose

AiE2517m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1504 - pAAV-AiE2187m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230663

Purpose

AiE2187m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1187 - pAAV-AiE2046m-minBG-FlpO-WPRE-HGHpA

Plasmid

#230578

Purpose

AiE2046m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1871 - pAAV-AiE2515m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#220677

Purpose

AiE2515m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1878 - pAAV-AiE2425m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214566

Purpose

AiE2425m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1873 - pAAV-AiE2499m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214564

Purpose

AiE2499m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1876 - pAAV-AiE2413m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214565

Purpose

AiE2413m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1879 - pAAV-AiE2570m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214567

Purpose

AiE2570m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-187 N-Tag GFP[1] L10 FKBP mCherry

Plasmid

#130003

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

FKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge