We narrowed to 1,197 results for: Aspa

1. HA-VHL Y98N-pBabe-puro

   Plasmid

   #19236

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   changed Tyrosin 98 to Asparagin

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS U6 WASp RNAi

   Plasmid

   #11775

   Depositor

   Jack Strominger

   Article

   Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

   Insert

   WASp RNAi (WAS Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pMTP130 (pTA106_TnsABC(Tn6900))

   Plasmid

   #164261

   Purpose

   Vector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.

   Depositor

   Joseph Peters

   Article

   Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

   Insert

   tnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   (tnsA) Changed Alanine 125 to Aspartic acid

   Promoter

   Lactose

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-VHL Y112N-pBabe-puro

   Plasmid

   #19238

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   changed Tyrosin 112 to Asparagin

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)

   Plasmid

   #122067

   Purpose

   Expressing Dhh1-PA

   Depositor

   Daniel Klionsky

   Article

   Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

   Insert

   Dhh1-PA (DHH1 Budding Yeast)
   

   Use

   Tags

   PA

   Expression

   Yeast

   Mutation

   Aspartic acid 195 to alanine; Glutamic acid 196 t…

   Promoter

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJCC_058 FnCas12a D917A

   Plasmid

   #171667

   Purpose

   For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   FnCas12a D917A
   

   Use

   Tags

   6X His and sortase A sorting motif

   Expression

   Bacterial

   Mutation

   changed aspartate 917 to alanine

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEos2-CD151-7

   Plasmid

   #57358

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   CD151 (CD151 Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pUHG10.3-TetO-Rs1

   Plasmid

   #16313

   Depositor

   Bruce Conklin

   Article

   Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

   Insert

   h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (RS1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Added FLAG tag; Changed Aspartic Acid 100 to Alan…

   Promoter

   Available since

   Jan. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-MBP-(CBX1)3-eReader

   Plasmid

   #135090

   Purpose

   Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered x3
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. NmMetQ N238A

    Plasmid

    #129648

    Purpose

    Expresses N238A mutation in the mature metQ gene from Neisseria meningitides, without the signal sequence

    Depositor

    Douglas Rees

    Article

    Nguyen et al Protein Sci. 2019 Jul 26. doi: 10.1002/p

    Insert

    metQ Gene
    

    Use

    Tags

    10x Histidine Tag, Enterokinase, and pelB signal …

    Expression

    Bacterial

    Mutation

    Changed Asparagine 238 to Alanine

    Promoter

    T7 Promoter

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-SpyTag002 DA-MBP

    Plasmid

    #102832

    Purpose

    Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyTag002 DA-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Reactive aspartic acid has been mutated to an ala…

    Promoter

    T7

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET30b GluBP600n

    Plasmid

    #119835

    Purpose

    Production of recombinant GluBP600n high affinity protein in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    GluBP600n
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3 Flag BimL (T56D)

    Plasmid

    #24237

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    BimL (T56D) (BCL2L11 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed threonine 56 to aspartic acid

    Promoter

    Available since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3 Flag Bmf (S74D)

    Plasmid

    #24259

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    Bmf (S87D) (BMF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Serine 74 to Aspartic Acid

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3 Flag BimL (S58D)

    Plasmid

    #24239

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    BimL (S58D) (BCL2L11 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed serine 58 to aspartic acid

    Promoter

    Available since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3 Flag BimL (S44D)

    Plasmid

    #24235

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    BimL (S44D) (BCL2L11 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed serine 44 to aspartic acid

    Promoter

    Available since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pCDNA3 T7 BimL (T56D)

    Plasmid

    #24532

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    Bim L(T56D) (BCL2L11 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Changed threonine 56 to aspartic acid

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pEGFP-STAG2-D299A

    Plasmid

    #31975

    Depositor

    Todd Waldman

    Article

    Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

    Insert

    human STAG2 cDNA (STAG2 Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    changed Aspartic acid 299 to Alanine

    Promoter

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pLANT-2b

    Plasmid

    #231487

    Purpose

    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

    Depositor

    Venkat Gopalan

    Inserts

    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    

    Use

    Tags

    His6 tag and His6-SUMO^NIT

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

    Promoter

    T7 promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Human DsRed-Monomer CASP9 C287A Mutant

    Plasmid

    #198469

    Purpose

    Expresses Human DsRed-Monomer CASP9 C287A Mutant Fusion Protein in Mammalian Cells

    Depositor

    Hannah Rabinowich

    Insert

    Human Caspase-9 C287A Mutant (CASP9 Human)
    

    Use

    Tags

    DsRed-monomer

    Expression

    Mammalian

    Mutation

    Changed Cysteine 287 to Alanine to eliminate prot…

    Promoter

    CMV

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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