We narrowed to 6,529 results for: human c myc

1. PB-PE SpG

   Plasmid

   #179081

   Purpose

   All-in-one prime editor piggyBac transposon, SpG variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R

   Promoter

   CAG

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-PE NG

   Plasmid

   #174554

   Purpose

   All-in-one prime editor piggyBac transposon, NG variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   L1111R, G1218R, E1219F, A1322R, R1335V, T1337R

   Promoter

   CAG

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-F-Med26-NTD

   Plasmid

   #49255

   Purpose

   Mammalian expression of flag-tagged Med26-NTD

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   Med 26 (MED26 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   NTD (aa 1-339)

   Promoter

   Available since

   Dec. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCW57-Cx32-IRES-GCaMP6s

   Plasmid

   #216795

   Purpose

   Inducible bicistronic lentiviral plasmid for the simultaneous expression of Connexin 32 and cytosolic GCaMP6s

   Depositor

   Fabio Mammano

   Article

   Tettey-Matey et al Cell Commun Signal. 2024 Dec 5;22(1):589

   Insert

   Connexin 32-IRES-GCaMP6s (GJB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCX-HO1-2A-EGFP

   Plasmid

   #74672

   Purpose

   Mammalian expression of HO1 and EGFP

   Depositor

   Roberto Giovannoni

   Article

   Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

   Insert

   HO1 (HMOX1 Human)
   

   Use

   Tags

   2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9-GFP-VPR

   Plasmid

   #181907

   Purpose

   Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIB-HIS-TEV-scCAK1

   Plasmid

   #177014

   Purpose

   Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
   

   Use

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMito-mCherry-FRB_IRES_FKBP(I)-GBPen

   Plasmid

   #128267

   Purpose

   Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 1x FKBP-GBPenhancer (GFP Nanobody with 1 FKBP domain).

   Depositor

   Stephen Royle

   Article

   Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

   Inserts

   MitoTrap
   FKBP(I)-GBPen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and IRES

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPK1520_puroR

   Plasmid

   #173901

   Purpose

   SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

   Depositor

   Ronald Cohn

   Article

   Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/U6

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEYFP-Pds1Δdestruction box(383)

    Plasmid

    #39848

    Depositor

    Jonathon Pines

    Article

    Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

    Insert

    Pds1 (PTTG1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Δdestruction box = ΔR61-N68

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-TAC-ERP2-(N-HA)KRAS_G12D

    Plasmid

    #201254

    Purpose

    Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

    Depositor

    Alexander Kleger

    Article

    Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

    Insert

    KRAS (KRAS Human)
    

    Use

    Piggybac transposon

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    changed Gly (G) 12 to Asp (D)

    Promoter

    TRE

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMito-mCherry-FRB_IRES_FKBP(III)-GBPen

    Plasmid

    #128268

    Purpose

    Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains).

    Depositor

    Stephen Royle

    Article

    Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

    Inserts

    MitoTrap
    FKBP(III)-GBPen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRosetta_v2

    Plasmid

    #136477

    Purpose

    Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. R777-E042 Hs.DUSP5-nostop

    Plasmid

    #70326

    Purpose

    Gateway ORF clone of human DUSP5 [NM_004419.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    DUSP5 (DUSP5 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    A220M (human SNP)

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSX2-pET

    Plasmid

    #21025

    Depositor

    Corey Largman

    Article

    Stelnicki et al Differentiation. 1997 Oct . 62(1):33-41.

    Insert

    MSX2 (Msx2 Mouse)
    

    Use

    Tags

    HIS and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

16. MSX1-pET

    Plasmid

    #21024

    Depositor

    Corey Largman

    Article

    Stelnicki et al Differentiation. 1997 Oct . 62(1):33-41.

    Insert

    MSX1 (Msx1 Mouse)
    

    Use

    Tags

    His and T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

17. AAVS1-TRE3G-EGFP

    Plasmid

    #52343

    Purpose

    AAVS1 donor vector for inducible gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRIPZ-Wt-PTEN

    Plasmid

    #206982

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of wild type PTEN

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-CAG-hrGFP

    Plasmid

    #52344

    Purpose

    AAVS1 donor vector for constitutive gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    hrGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. p8092 LentiCRISPR v2 sgNT-1

    Plasmid

    #163315

    Purpose

    Expresses Cas9 and a non-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

    Insert

    sgNT-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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