We narrowed to 6,078 results for: nls

1. pAAV2-CAG-3xNLS-mScarlet

   Plasmid

   #191098

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-mScarlet
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   Optimized to human codon usage

   Promoter

   CMV enhancer and CAG

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-EFS-MCP-3XBFPnls

   Plasmid

   #75384

   Purpose

   MCP-3XBFPnls

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   MCP
   

   Use

   Lentiviral

   Tags

   3XBFP

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-NLS-miRFP670nano-miRFP670

   Plasmid

   #216131

   Purpose

   Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLS-mCherry-LEXY (pDN122)

   Plasmid

   #72655

   Purpose

   Blue light-inducible nuclear export of mCherry

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

   Insert

   NLS-mCherry-LEXY
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-His:dCas9:nls:10P3

   Plasmid

   #135982

   Purpose

   Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   dCas9 fused to 10 copies of the P3 coiled-coil
   

   Use

   CRISPR

   Tags

   His

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. CuO-LacI-NLS–SNAPf

   Plasmid

   #227368

   Purpose

   Lentiviral expression of LacI–SNAP

   Depositor

   David Pellman

   Article

   Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

   Insert

   LacI NLS SNAPf
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-NLS-actin-R62D

   Plasmid

   #118381

   Purpose

   Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Tags

   Expression

   Mutation

   NLS and R62D

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1 Utr230-EGFP-3XNLS

   Plasmid

   #58466

   Purpose

   Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

   Inserts

   Utr230 with 3X glycine linker (UTRN Human)
   3X NLS with 3X stop codons
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1 Lifeact-EGFP-2XNLS

   Plasmid

   #58467

   Purpose

   Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

   Inserts

   Lifeact
   2X NLS with 2X stop codons
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiGuide-puro-NLS-GFP

    Plasmid

    #185473

    Purpose

    Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pMSCV-NLS-CBRed-d2-hygro

    Plasmid

    #139196

    Purpose

    non-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puro

    Depositor

    Matthew Lazzara

    Article

    Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

    Insert

    CBRed
    

    Use

    Luciferase and Retroviral

    Tags

    d2 PEST domain and nuclear localization signal (N…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-Cas9-NLS-6xHis

    Plasmid

    #62933

    Purpose

    Expression of Cas9-NLS-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9-NLS-6xHis
    

    Use

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid

    #60365

    Purpose

    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    RNase HI
    

    Use

    Tags

    NLS from SV40 T antigen and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. NLS-tdPCP-GFP-VPR

    Plasmid

    #183936

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-GFP-VPR
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDN34-BiNLS-hYAP1-1delta

    Plasmid

    #169471

    Purpose

    expresses optoYAP in mammalian cells

    Depositor

    Timothy Saunders

    Article

    Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

    Insert

    YAP1-1delta (YAP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUbC-nls-ha-stdMCP-stdGFP

    Plasmid

    #98916

    Purpose

    synonymized tandem dimer MCP fused to synonymized tandem dimer GFP

    Depositor

    Robert Singer

    Article

    Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi

    Insert

    stdMCP-stdGFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-EFS-MCP-HALOnls

    Plasmid

    #121937

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    MCP-HALOnls
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV_CAG_Kinprola_PKA-mEGFP-NLS3__WPRE-SV40

    Plasmid

    #233365

    Purpose

    CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for mammalian cell expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    Kinprola_PKA-mEGFP-NLS3X
    

    Use

    AAV

    Tags

    mEGFP-NLS3X

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV2-CAG-3xNLS-AausFP1

    Plasmid

    #191096

    Purpose

    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-AausFP1
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    Optimized to Human codon usage

    Promoter

    CMV enhancer and CAG

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGTag-NLS-eGFP-SV40

    Plasmid

    #117811

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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