We narrowed to 6,177 results for: nls

1. Lp-BFPnls-LP-Cherry-T2A-NTR2.0

   Plasmid

   #219785

   Purpose

   Used for generating a transgenic line for inducing NTR2.0 expression in Cre positive cells after conversion

   Depositor

   Prayag Murawala

   Article

   García-García et al bioRxiv 2025.03.30.645595; doi: 10.1101/

   Insert

   mTagBFP2
   

   Tags

   3x SV40 NLS

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-EGFP-NLS

   Plasmid

   #165441

   Purpose

   Expresses nuclear EGFP from CMV promoter

   Depositor

   Esteban Engel

   Article

   Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

   Insert

   EGFP-NLS
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-NLS-Cas9-6xHis

   Plasmid

   #62934

   Purpose

   Expression of NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   NLS-Cas9-6xHis
   

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. CuO-LacI-NLS–SNAPf

   Plasmid

   #227368

   Purpose

   Lentiviral expression of LacI–SNAP

   Depositor

   David Pellman

   Article

   Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

   Insert

   LacI NLS SNAPf
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

   Plasmid

   #196702

   Purpose

   Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 wild type
   

   Expression

   Mammalian

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dCas13-inactive M3nls

   Plasmid

   #157854

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
   

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGs-NLS-TIR1_P2A_NES-TIR1

   Plasmid

   #117699

   Purpose

   Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

   Depositor

   Joerg Mansfeld

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Inserts

   TRANSPORT INHIBITOR RESPONSE 1
   TRANSPORT INHIBITOR RESPONSE 1
   

   Tags

   HA tag, Myc-NES, and NLS

   Expression

   Mammalian

   Mutation

   codon optimized for murine expression

   Promoter

   None, 2A peptide from porcine teschovirus-1 polyp…

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. CSII-prEF1a-mCherry-3xNLS

   Plasmid

   #125262

   Purpose

   Fluorescent reporter for cell size

   Depositor

   Jan Skotheim

   Article

   Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

   Insert

   mCherry-3xNLS
   

   Use

   Lentiviral

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRE-mClover3-TDP-43ΔNLS

   Plasmid

   #214917

   Purpose

   inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   TDP-43ΔNLS (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   mClover3

   Mutation

   mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-XBP1 mNeonGreen NLS

    Plasmid

    #115968

    Purpose

    ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    X-box binding protein 1 (XBP1 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-MBP-2xNLS-tdTomato

    Plasmid

    #104054

    Purpose

    An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    2xNLS-tdTomato
    

    Use

    AAV

    Tags

    NLS

    Promoter

    MBP

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-ATF4 mScarlet NLS

    Plasmid

    #115969

    Purpose

    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    activating transcription factor 4 (ATF4 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-EFS-MCP-3XBFPnls

    Plasmid

    #75384

    Purpose

    MCP-3XBFPnls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    3XBFP

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-ArgiNLS-AausFP1

    Plasmid

    #220595

    Purpose

    Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    AausFP1
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRE-TDP-43ΔNLS-mClover3

    Plasmid

    #214916

    Purpose

    inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    TDP-43ΔNLS (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Mutation

    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-mDlx-NLS-mRuby2

    Plasmid

    #99130

    Purpose

    An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV8, and AAV9

    Insert

    NLS-mRuby2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    mDLX5/6

    Available Since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. YFP NLS Beta-Actin

    Plasmid

    #60613

    Purpose

    Encodes YFP and NLS tagged beta-actin

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-NLS-actin-R62D

    Plasmid

    #118381

    Purpose

    Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Mutation

    NLS and R62D

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti-NLS-miRFP670nano-miRFP670

    Plasmid

    #216131

    Purpose

    Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.2 TDP-43 NLS1 YFP

    Plasmid

    #84912

    Purpose

    Mammalian expresion of TDP-43 NLS1 YFP

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A

    Promoter

    CMV

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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