We narrowed to 11,803 results for: nsf

1. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

   Plasmid

   #112211

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   C-terminus of dCas9
   

   Use

   AAV

   Mutation

   D10A

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4xhyb PylT sfGFP 150TAG

   Plasmid

   #154771

   Purpose

   plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TAG

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMS2117

   Plasmid

   #49142

   Purpose

   AAV plasmid with smCBA promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-smCBA-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   smCBA

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAAVsc.U6-sgFah.CMV/CB-EGFP

   Plasmid

   #121508

   Purpose

   Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   sgFah
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMflT-o4

   Plasmid

   #101312

   Purpose

   Contains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Insert

   oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE

   Plasmid

   #118279

   Purpose

   Expresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cells

   Depositor

   Dietmar Schmitz

   Article

   Wozny et al Front Cell Neurosci. 2018 Oct 2;12:337.

   Inserts

   mRuby2
   ChR2(H134R)
   

   Use

   AAV and Cre/Lox

   Tags

   SV40 NLS and YFP

   Promoter

   EF1 alpha

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-DR1-NRSE-RFP

   Plasmid

   #22927

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   peroxisome proliferator activated receptor gamma binding site promoter with neuron-restrictive silencer element with minimal CMV
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA-G-intron(Neo)-T(FLLFLNL)

   Plasmid

   #40020

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tdT-3Myc
   beta-globin intron
   Neo
   

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available Since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMS2131

   Plasmid

   #111895

   Purpose

   ssAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Type

   Empty backbone

   Use

   AAV

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

    Plasmid

    #63886

    Purpose

    An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized GCaMP3(wt)
    

    Use

    AAV

    Promoter

    human SYN1

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. P12p-bio

    Plasmid

    #47726

    Purpose

    Expresses enzymatically monobiotinylated full-length P12p ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised P12p
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Hyg-FHIT

    Plasmid

    #16410

    Depositor

    Bert Vogelstein

    Article

    Kohli et al Nucleic Acids Res. 2004 . 32(1):e3.

    Insert

    FHIT Hyg targeting (FHIT Human)
    

    Use

    AAV; Adeno-associated viral

    Expression

    Mammalian

    Available Since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

13. RON3-bio

    Plasmid

    #47799

    Purpose

    Expresses enzymatically monobiotinylated full-length RON3 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RON3
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTNos:BASTA:PNos (GB0023)

    Plasmid

    #68211

    Purpose

    Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    bar gene
    

    Use

    Unspecified

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    PNos

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBT250_(pCA-G-intron(Neo)-tTA2)

    Plasmid

    #36876

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. RAP2-bio

    Plasmid

    #47795

    Purpose

    Expresses enzymatically monobiotinylated full-length RAP2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RAP2
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLTR PKC zeta

    Plasmid

    #8421

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Mouse Protein Kinase C zeta (Prkcz Mouse)
    

    Expression

    Mammalian

    Available Since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

    Plasmid

    #124859

    Purpose

    Mutagenesis of Slc17a7

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a7 (Slc17a7 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-MBD

    Plasmid

    #114371

    Purpose

    expresses Flpo recombinase-dependent GtACR2-MBD, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-MBD (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and MBD

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. SPATR-bio

    Plasmid

    #47782

    Purpose

    Expresses enzymatically monobiotinylated full-length SPATR with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised SPATR
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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