We narrowed to 10,356 results for: plasmids 101
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Plasmid#230917PurposeTested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.DepositorInsertHuman COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
UseLuciferaseTagsLuciferase-luc2pExpressionBacterial and MammalianMutationCOL1A1 promoter with mutant potential SMAD bindin…PromoterCOL1A1Available SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-KMS
Plasmid#188038PurposePolycistronic human KLF4, MYC and SOX2 were cloned into the pMXs vectorDepositorUseRetroviralTagsExpressionMammalianMutationPromoterAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-OKM
Plasmid#136554PurposeDox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Klf4, cMycDepositorUseLentiviralTagsExpressionBacterialMutationPromotertetO-miniCMV (dox-inducible)Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-KSM
Plasmid#136553PurposeDox-inducible polycistronic reprogramming lentiviral vector expressing mouse Klf4, Sox2, cMycDepositorUseLentiviralTagsExpressionBacterialMutationPromotertetO-miniCMV (dox-inducible)Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-OSM
Plasmid#136555PurposeDox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, cMycDepositorUseLentiviralTagsExpressionBacterialMutationPromotertetO-miniCMV (dox-inducible)Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGL4.11-COL1A1mTEAD
Plasmid#230916PurposeComparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.DepositorInsertHuman COL1A1 promoter with mutant TEAD elements (COL1A1 Human)
UseLuciferaseTagsLuciferase- luc2pExpressionBacterial and MammalianMutationmutant potential TEAD bind elements AGGAAT to CTG…PromoterCOL1A1Available SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEMS1371
Plasmid#29172PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle99 (GRP Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable SinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1374
Plasmid#29175PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle102 (GRP Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable SinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
PGL4.11-COL1A1double mutants
Plasmid#230918PurposeDouble mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.DepositorInsertHuman CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
UseLuciferaseTagsLuciferase-luc2pExpressionBacterial and MammalianMutationCOL1A1 promoter with mutant SMAD and TEAD binding…PromoterCOL1A1 with mutant SMAD and TEAD elementsAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_CORO1B:1-489
Plasmid#211196PurposeMammalian expression of full-length human CORO1B. N-terminal HiBiT tag.DepositorInsertCORO1B:M1-A489 (CORO1B Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_PREB:1-417
Plasmid#211131PurposeMammalian expression of full-length human PREB. N-terminal HiBiT tag.DepositorInsertPREB:M1-L417 (PREB Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_FBXW12:1-394
Plasmid#211208PurposeMammalian expression of full-length human FBXW12 isoform 2. N-terminal HiBiT tag.DepositorInsertFBXW12 :M1-T394 (FBXW12 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationisoform 2PromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_PREB:1-417
Plasmid#211172PurposeMammalian expression of full-length human PREB. C-terminal HiBiT tag.DepositorInsertPREB:M1-L417 (PREB Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_FBXW12:1-394
Plasmid#211282PurposeMammalian expression of full-length human FBXW12 isoform 2. C-terminal HiBiT tag.DepositorInsertFBXW12 :M1-T394 (FBXW12 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationisoform 2PromoterCMVAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_CORO1B:1-489
Plasmid#211270PurposeMammalian expression of full-length human CORO1B. C-terminal HiBiT tag.DepositorInsertCORO1B:M1-A489 (CORO1B Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_CORO1B:1-489
Plasmid#210906PurposeInsect expression of full-length human CORO1B. N-terminal 6X His tag with TEV cleavage.DepositorInsertCORO1B:M1-A489 (CORO1B Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-LIC_GRWD1:1-446
Plasmid#210876PurposeInsect expression of full-length human GRWD1. N-terminal 6X His tag with thrombin protease cleavage site.DepositorInsertGRWD1:M1-V446 (GRWD1 Human)
UseTagsMGSSHHHHHHSSGLVPRGSExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR
Plasmid#210525PurposeAAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsmtagBFP-KASH
U6-gRNA
UseAAVTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR
Plasmid#224435PurposeAAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsmScarlet-KASH
U6-gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR
Plasmid#213481PurposeAAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsGFP-KASH
U6-gRNA
UseAAVTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only